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Aptamer-Based Dual-Functional Probe for Rapid and Specific Counting and Imaging of MCF-7 Cells

Development of multimodal detection technologies for accurate diagnosis of cancer at early stages is in great demand. In this work, we report a novel approach using an aptamer-based dual-functional probe for rapid, sensitive, and specific counting and visualization of MCF-7 cells by inductively coupled plasma–mass spectrometry (ICP-MS) and fluorescence imaging. The probe consists of a recognition unit of aptamer to catch cancer cells specifically, a fluorescent dye (FAM) moiety for fluorescence resonance energy transfer (FRET)-based “off-on” fluorescence imaging as well as gold nanoparticles (Au NPs) tag for both ICP-MS quantification and fluorescence quenching. Due to the signal amplification effect and low spectral interference of Au NPs in ICP-MS, an excellent linearity and sensitivity were achieved. Accordingly, a limit of detection of 81 MCF-7 cells and a relative standard deviation of 5.6% (800 cells, n = 7) were obtained. The dynamic linear range was 2 × 102 to 1.2 × 104 cells, and the recoveries i...

Authors:   Bin Yang; Beibei Chen; Man He; Xiao Yin; Chi Xu; Bin Hu
Journal:   Analytical Chemistry
Year:   2018
DOI:   10.1021/acs.analchem.7b04927
Publication date:   19-Jan-2018
Facts, background information, dossiers
  • standard deviation
  • spectrometry
  • interference
  • diagnosis
  • cancer cells
  • cancer
  • amplification
More about American Chemical Society Publications
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