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Human teeth biobank: Microbiological analysis of the teeth storage solution

Abstract

The cross‐infections may occur during handling of dental elements, affecting the health of dental practitioners and researchers. This study aimed to analyze the influence of the storage medium temperature on the bacterial contingent of the human teeth used for research purposes. Thirty human teeth were donated to the Human Teeth Biobank immediately after extraction. The teeth were cleaned with tap water and neutral soap. The teeth were randomly distributed according to the temperature of the storage solution (deionized water): at 4 °C (refrigerator) or at −10 °C (freezer) and were stored individually in sterile vials during 60 days. After this period, a microbiological analysis (CFU/mL) of the storage solutions was performed and teeth were submitted to SEM analysis. Data were analyzed by Kruskal–Wallis test followed by Dunn's post‐test (p ≤ .05). Total aerobic bacteria ranged from 5.8 to 8.4 log10 CFU/mL for refrigerated solution and from 1.9 to 8.5 log10 CFU/mL for frozen solution. No statistical differences were found between the storage solutions (p > .05). The counts of Streptococcus spp., Lactobacillus spp., and Staphylococcus spp. were similar for both storage solutions (p > .05). SEM analysis showed spiral‐ and rod‐shaped bacteria attached on teeth stored under 4 °C, which may suggest the presence of Treponema spp. and Lactobacillus spp. Similar morphological forms were found on teeth stored under −10 °C. A biofilm organized in honeycomb‐like form was found in the frozen teeth. Cocci were eventually found in all the samples. It was concluded that bacterial growth and survival were not influenced by the temperature of the teeth storage solution.

  • There is no consensus about the most indicated method to store human teeth.
  • Groups showed similar morphological findings, but organized honeycomb‐like biofilms were found only for the teeth stored under −10 °C.
  • The bacterial growth and survival were not influenced by the temperature.

Authors:   Fabiana Almeida Curylofo‐Zotti, Francine Lorencetti‐Silva, Jéssica de Almeida Coelho, Rachel Maciel Monteiro, Evandro Watanabe, Silmara Aparecida Milori Corona
Journal:   Microscopy Research and Technique
Year:   2018
Pages:   n/a
DOI:   10.1002/jemt.22984
Publication date:   17-Jan-2018
Facts, background information, dossiers
  • Total
  • temperature
  • Streptococcus
  • Staphylococcus
  • samples
  • Research
  • microbiological analyses
  • Lactobacillus
  • biofilms
  • bacteria
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