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Dual Signaling DNA Electrochemistry: An Approach To Understand DNA Interfaces

Electrochemical DNA biosensors composed of a redox marker modified nucleic acid probe tethered to a solid electrode is a common experimental construct for detecting DNA and RNA targets, proteins, inorganic ions, and even small molecules. This class of biosensors generally relies on the binding-induced conformational changes in the distance of the redox marker relative to the electrode surface such that the charge transfer is altered. The conventional design is to attach the redox species to the distal end of a surface-bound nucleic acid strand. Here we show the impact of the position of the redox marker, whether on the distal or proximal end of the DNA monolayer, on the DNA interface electrochemistry. Somewhat unexpectedly, greater currents were obtained when the redox molecules were located on the distal end of the surface-bound DNA monolayer, notionally furthest away from the electrode, compared with currents when the redox species were located on the proximal end, close to the electrode. Our results su...

Authors:   Saimon Moraes Silva; Roya Tavallaie; Vinicius R. Gonçales; Robert H. Utama; Mehran B. Kashi; D. Brynn Hibbert; Richard D. Tilley; J. Justin Gooding
Journal:   Langmuir
Year:   2018
DOI:   10.1021/acs.langmuir.7b02787
Publication date:   18-Jan-2018
Facts, background information, dossiers
  • DNA
  • small molecules
  • RNA
  • proteins
  • molecules
  • ions
  • charge transfer
  • biosensors
More about American Chemical Society Publications
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