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Protein–Substrate Adhesion in Microcontact Printing Regulates Cell Behavior

Microcontact printing (μCP) is widely used to create patterns of biomolecules essential for studies of cell mechanics, migration, and tissue engineering. However, different types of μCPs may create micropatterns with varied protein–substrate adhesion, which may change cell behaviors and pose uncertainty in result interpretation. Here, we characterize two μCP methods for coating extracellular matrix (ECM) proteins (stamp-off and covalent bond) and demonstrate for the first time the important role of protein–substrate adhesion in determining cell behavior. We found that, as compared to cells with weaker traction force (e.g., endothelial cells), cells with strong traction force (e.g., vascular smooth muscle cells) may delaminate the ECM patterns, which reduced cell viability as a result. Importantly, such ECM delamination was observed on patterns by stamp-off but not on the patterns by covalent bonds. Further comparisons of the displacement of the ECM patterns between the normal VSMCs and the force-reduced V...

Authors:   Shuhuan Hu; Ting-Hsuan Chen; Yanhua Zhao; Zuankai Wang; Raymond H. W. Lam
Journal:   Langmuir
Year:   2018
DOI:   10.1021/acs.langmuir.7b02935
Publication date:   18-Jan-2018
Facts, background information, dossiers
  • proteins
  • extracellular matrix
  • endothelial cells
  • cells
  • cell viability
  • biomolecules
More about American Chemical Society Publications
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