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Insights into the MALDI Process after Matrix Deposition by Sublimation Using 3D ToF-SIMS Imaging

Imaging mass spectrometry (IMS) has become a powerful tool to characterize the spatial distribution of biomolecules in thin tissue sections. In the case of matrix-assisted laser desorption ionization (MALDI) IMS, homogeneous matrix deposition is critical to produce high-quality ion images, and sublimation in particular has shown to be an excellent matrix deposition method for the imaging of lipids. Matrix deposition by sublimation is, however, a completely solvent-free system, which ought to prevent the mixing of matrix and analytes thought to be necessary for successful MALDI. Using 3D time-of-flight secondary ion imaging mass spectrometry, we have studied the matrix–tissue interface in 3D with high resolution to understand the MALDI process of lipids after matrix deposition by sublimation. There is a strong indication that diffusion is the process by which lipids migrate from the tissue to the matrix layer. We show that triacylglycerols and phospholipids have a delayed migratory trend as compared to dia...

Authors:   Sebastiaan Van Nuffel; Nicolas Elie; Ethan Yang; Julius Nouet; David Touboul; Pierre Chaurand; Alain Brunelle
Journal:   Analytical Chemistry
Year:   2018
DOI:   10.1021/acs.analchem.7b03993
Publication date:   17-Jan-2018
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