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Gold nanoparticle-decorated reduced-graphene oxide targeting anti hepatitis B virus core antigen

Publication date:

August 2018


Source:Bioelectrochemistry, Volume 122

Author(s): Mohamad Farid Abd Muain, Kooi Hoong Cheo, Muhamad Nadzmi Omar, Amir Syahir Amir Hamzah, Hong Ngee Lim, Abu Bakar Salleh, Wen Siang Tan, Asilah Ahmad Tajudin

Hepatitis B virus core antigen (HBcAg) is the major structural protein of hepatitis B virus (HBV). The presence of anti-HBcAg antibody in a blood serum indicates that a person has been exposed to HBV. This study demonstrated that the immobilization of HBcAg onto the gold nanoparticles-decorated reduced graphene oxide (rGO-en-AuNPs) nanocomposite could be used as an antigen-functionalized surface to sense the presence of anti-HBcAg. The modified rGO-en-AuNPs/HBcAg was then allowed to undergo impedimetric detection of anti-HBcAg with anti-estradiol antibody and bovine serum albumin as the interferences. Upon successful detection of anti-HBcAg in spiked buffer samples, impedimetric detection of the antibody was then further carried out in spiked human serum samples. The electrochemical response showed a linear relationship between electron transfer resistance and the concentration of anti-HBcAg ranging from 3.91ngmL−1 to 125.00ngmL−1 with lowest limit of detection (LOD) of 3.80ngmL−1 at 3σm−1. This established method exhibits potential as a fast and convenient way to detect anti-HBcAg.
Graphical abstract




Authors:   Author(s): Mohamad Farid Abd Muain, Kooi Hoong Cheo, Muhamad Nadzmi Omar, Amir Syahir Amir Hamzah, Hong Ngee Lim, Abu Bakar Salleh, Wen Siang Tan, Asilah Ahmad Tajudin
Journal:   Bioelectrochemistry
Volume:   122
Year:   2018
Pages:   199
DOI:   10.1016/j.bioelechem.2018.04.004
Publication date:   15-Apr-2018
Facts, background information, dossiers
  • samples
  • hepatitis B
  • graphene oxide
  • concentration
  • bovine serum albumin
  • blood
More about Elsevier
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