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Sensors, Vol. 18, Pages 354: Label-Free Aptasensor for Lysozyme Detection Using Electrochemical Impedance Spectroscopy

Sensors, Vol. 18, Pages 354: Label-Free Aptasensor for Lysozyme Detection Using Electrochemical Impedance Spectroscopy

Sensors doi: 10.3390/s18020354

Authors: Dionisia Ortiz-Aguayo Manel del Valle

This research develops a label-free aptamer biosensor (aptasensor) based on graphite-epoxy composite electrodes (GECs) for the detection of lysozyme protein using Electrochemical Impedance Spectroscopy (EIS) technique. The chosen immobilization technique was based on covalent bonding using carbodiimide chemistry; for this purpose, carboxylic moieties were first generated on the graphite by electrochemical grafting. The detection was performed using [Fe(CN)6]3−/[Fe(CN)6]4− as redox probe. After recording the frequency response, values were fitted to its electric model using the principle of equivalent circuits. The aptasensor showed a linear response up to 5 µM for lysozyme and a limit of detection of 1.67 µM. The sensitivity of the established method was 0.090 µM−1 in relative charge transfer resistance values. The interference response by main proteins, such as bovine serum albumin and cytochrome c, has been also characterized. To finally verify the performance of the developed aptasensor, it was applied to wine analysis.

Authors:   Ortiz-Aguayo, Dionisia ; del Valle, Manel
Journal:   Sensors
Volume:   18
edition:   2
Year:   2018
Pages:   354
DOI:   10.3390/s18020354
Publication date:   26-Jan-2018
Facts, background information, dossiers
  • wine analyses
  • Research
  • proteins
  • charge transfer
  • bovine serum albumin
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