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Molecules, Vol. 23, Pages 1273: Antibodies towards Tyrosine Amyloid-Like Fibrils Allow Toxicity Modulation and Cellular Imaging of the Assemblies

Molecules, Vol. 23, Pages 1273: Antibodies towards Tyrosine Amyloid-Like Fibrils Allow Toxicity Modulation and Cellular Imaging of the Assemblies

Molecules doi: 10.3390/molecules23061273

Authors: Dor Zaguri Topaz Kreiser Shira Shaham-Niv Ehud Gazit

The amino acid tyrosine forms cytotoxic amyloid-like fibrils by molecular self-assembly. However, the production of antibodies towards tyrosine assemblies, reflecting their presentation to the immune system, was not demonstrated yet. Here, we describe the production of antibodies that specifically recognize tyrosine in its fibrillated form. The antibodies were demonstrated to specifically bind self-assembled tyrosine, in contrast to its non-aggregated form or disintegrated fibrils. The antibodies could be used for immunostaining of tyrosine fibrils in cultured cells. Furthermore, confocal microscopy allowed a demonstration of the intracellular presence of the metabolite amyloids in a neuroblastoma cell model. Finally, pre-incubation of tyrosine fibrils with the antibodies resulted in significant reduction in their cytotoxicity. Taken together, we provide an experimental proof for the immunogenicity of tyrosine amyloid fibrillary assemblies. These specific antibodies against tyrosine structures could be further used as a research tool to study the dynamics, toxicity and cellular localization of the assemblies.

Authors:   Zaguri, Dor ; Kreiser, Topaz ; Shaham-Niv, Shira ; Gazit, Ehud
Journal:   Molecules
Volume:   23
edition:   6
Year:   2018
Pages:   1273
DOI:   10.3390/molecules23061273
Publication date:   26-May-2018
Facts, background information, dossiers
  • antibodies
  • tyrosine
  • molecules
  • production
  • neuroblastoma
  • immunostaining
  • immunogenicity
  • immune system
  • fibrils
  • cytotoxicity
  • confocal microscopy
  • cellular imaging
  • cells
  • amyloids
More about Molecular Diversity Preservation International
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