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In Vitro Fertilization in Mice

The success of in vitro fertilization is strongly influenced by genetic background, handling of sperm and oocytes, and culture conditions. This protocol promotes enhanced rates of fertilization by using preincubation medium for sperm containing methyl-β-cyclodextrin (MBCD) and fertilization medium with high calcium and reduced glutathione (GSH).

Authors:   Takeo, T., Nakagata, N.
Journal:   CSH Protocols
Volume:   2018
edition:   6
Year:   2018
Publication date:   01-Jun-2018
Facts, background information, dossiers
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