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Human plasma and serum extracellular small RNA reference profiles and their clinical utility [Medical Sciences]

Nucleic acids mediate storage and expression of genetic information. Extracellular DNA (exDNA) and exRNA are traces of nucleic acids released from cells into the extracellular environment. Their use as disease biomarkers has been limited by technical challenges in their isolation caused by abundant RNA- and DNA-degrading enzymes in biofluids. Using isolation protocols developed especially for biofluids, we generated plasma and serum exRNA reference profiles from 13 healthy volunteers over time and determined the effect of critical clinical parameters such as gender and fasting. Surprisingly, we encountered one participant with dramatically increased endocrine-origin exRNA contributions stable over 1 year and detectable in all of his samples, thereby demonstrating the robustness of this approach and the clinical potential of circulating RNAs as biomarkers.

Authors:   Klaas E. A. Max; Karl Bertram; Kemal Marc Akat; Kimberly A. Bogardus; Jenny Li; Pavel Morozov; Iddo Z. Ben-Dov; Xin Li; Zachary R. Weiss; Azadeh Azizian; Anuoluwapo Sopeyin; Thomas G. Diacovo; Catherine Adamidi; Zev Williams; Thomas Tuschl
Journal:   Proceedings of the National Academy of Sciences current issue
Volume:   115
edition:   23
Year:   2018
Pages:   E5334
DOI:   10.1073/pnas.1714397115
Publication date:   05-Jun-2018
Facts, background information, dossiers
  • biomarkers
  • small RNA
  • samples
  • RNA
  • Reference
  • Expression
  • environment
More about Proceedings of the National Academy of Sciences
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