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Molecules, Vol. 23, Pages 1418: Plagiochiline A Inhibits Cytokinetic Abscission and Induces Cell Death

Molecules, Vol. 23, Pages 1418: Plagiochiline A Inhibits Cytokinetic Abscission and Induces Cell Death

Molecules doi: 10.3390/molecules23061418

Authors: Nicole S. Stivers Ashraful Islam Elsa M. Reyes-Reyes Lavona K. Casson José C. Aponte Abraham J. Vaisberg Gerald B. Hammond Paula J. Bates

We previously reported on the isolation and biological activities of plagiochiline A (1), a 2,3-secoaromadendrane-type sesquiterpenoid from the Peruvian medicinal plant, Plagiochila disticha. This compound was found to have antiproliferative effects on a variety of solid tumor cell lines, as well as several leukemia cell lines. Other researchers have also noted the cytotoxicity of plagiochiline A (isolated from different plant species), but there are no prior reports regarding the mechanism for this bioactivity. Here, we have evaluated the effects of plagiochiline A on cell cycle progression in DU145 prostate cancer cells. A cell cycle analysis indicated that plagiochiline A caused a significant increase in the percentage of cells in the G2/M phase when compared with control cells. When cells were stained and observed by fluorescence microscopy to examine progress through the mitotic phase, we found a significant increase in the proportion of cells with features of late cytokinesis (cells connected by intercellular bridges) in the plagiochiline A-treated samples. These results suggest that plagiochiline A inhibits cell division by preventing completion of cytokinesis, particularly at the final abscission stage. We also determined that plagiochiline A reduces DU145 cell survival in clonogenic assays and that it induces substantial cell death in these cells.

Authors:   Stivers, Nicole S.; Islam, Ashraful ; Reyes-Reyes, Elsa M.; Casson, Lavona K.; Aponte, José C.; Vaisberg, Abraham J.; Hammond, Gerald B.; Bates, Paula J.
Journal:   Molecules
Volume:   23
edition:   6
Year:   2018
Pages:   1418
DOI:   10.3390/molecules23061418
Publication date:   12-Jun-2018
Facts, background information, dossiers
  • cells
  • molecules
  • cell lines
  • cell death
  • cell cycle
  • samples
  • cytotoxicity
  • cell division
  • bioactivity
More about Molecular Diversity Preservation International
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