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IJMS, Vol. 19, Pages 1828: The Antioxidant and Anti-Inflammatory Activities of 8-Hydroxydaidzein (8-HD) in Activated Macrophage-Like RAW264.7 Cells

IJMS, Vol. 19, Pages 1828: The Antioxidant and Anti-Inflammatory Activities of 8-Hydroxydaidzein (8-HD) in Activated Macrophage-Like RAW264.7 Cells

International Journal of Molecular Sciences doi: 10.3390/ijms19071828

Authors: Eunji Kim Young-Gyu Kang Ji Hye Kim Yong-Jin Kim Tae Ryong Lee Jongsung Lee Donghyun Kim Jae Youl Cho

8-Hydroxydaidzein (8-HD) is a daidzein metabolite isolated from soybeans. This compound has been studied for its anti-proliferation, depigmentation, and antioxidant activities. However, the anti-inflammatory activities of 8-HD are not well-understood. Through its antioxidant effects in ABTS and DPPH assays, 8-HD reduces the production of sodium nitroprusside (SNP)-induced radical oxygen species (ROS). By triggering various Toll-like receptors (TLRs), 8-HD suppresses the inflammatory mediator nitric oxide (NO) without cytotoxicity. We examined the regulatory mechanism of 8-HD in lipopolysaccharide (LPS)-induced conditions. We found that 8-HD diminishes inflammatory gene expression (e.g., inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, and tumor necrosis factor (TNF)-α) by regulating the transcriptional activities of nuclear factor (NF)-κB and activator protein 1 (AP-1). To find the potential targets of 8-HD, signaling pathways were investigated by immunoblotting analyses. These analyses revealed that 8-HD inhibits the activation of TAK1 and that phosphorylated levels of downstream molecules decrease in sequence. Together, our results demonstrate the antioxidant and anti-inflammatory actions of 8-HD and suggest its potential use in cosmetics or anti-inflammatory drugs.

Authors:   Kim, Eunji ; Kang, Young-Gyu ; Kim, Ji Hye; Kim, Yong-Jin ; Lee, Tae Ryong; Lee, Jongsung ; Kim, Donghyun ; Cho, Jae Youl
Journal:   International Journal of Molecular Sciences
Volume:   19
edition:   7
Year:   2018
Pages:   1828
DOI:   10.3390/ijms19071828
Publication date:   21-Jun-2018
Facts, background information, dossiers
  • production
  • oxygen
  • nitric oxide synthase
  • immunoblotting
  • gene expression
  • drugs
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