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Molecules, Vol. 23, Pages 1504: Rapid Detection of Six Glucocorticoids Added Illegally to Dietary Supplements by Combining TLC with Spot-Concentrated Raman Scattering

Molecules, Vol. 23, Pages 1504: Rapid Detection of Six Glucocorticoids Added Illegally to Dietary Supplements by Combining TLC with Spot-Concentrated Raman Scattering

Molecules doi: 10.3390/molecules23071504

Authors: Li Li Xin Liang Tao Xu Feng Xu Wei Dong

The objective of this study was to establish a novel method for rapid detection of six glucocorticoids (prednisone, prednisone acetate, prednisolone, hydrocortisone, hydrocortisone acetate, and dexamethasone) added illegally in dietary supplements simultaneously by combining thin layer chromatography (TLC) with spot-concentrated Raman scattering (SCRS). The doping ingredients were separated by TLC, and viewed and located with UV light (254 nm), enriched by chromatography, then Raman spectra were directly detected by a Raman Imagine microscope with 780 nm laser source. This method had complementary advantages of TLC and Raman spectroscopy, which enhanced the specificity of the test results. The limit of detection (LOD) of the reference substances were 4 μg, 4 μg, 4 μg, 6 μg, 6 μg, and 4 μg, respectively. The method was used to study the six glucocorticoids added illegally in five dietary supplements. Fake drugs had been detected. The study showed that the TLC-SCRS method is simple, rapid, specific, sensitive, and reliable. The method could be used for effective separation and detection of six chemical components used in dietary supplement products, and would have good prospects for on-site qualitative screening of dietary supplement products for adulterants.

Authors:   Li, Li ; Liang, Xin ; Xu, Tao ; Xu, Feng ; Dong, Wei
Journal:   Molecules
Volume:   23
edition:   7
Year:   2018
Pages:   1504
DOI:   10.3390/molecules23071504
Publication date:   21-Jun-2018
Facts, background information, dossiers
  • dietary supplements
  • prednisone
  • molecules
  • hydrocortisone
  • glucocorticoids
  • ultraviolet light
More about Molecular Diversity Preservation International
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