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Materials, Vol. 11, Pages 1098: Human Dental Pulp Stem Cells Exhibit Different Biological Behaviours in Response to Commercial Bleaching Products

Materials, Vol. 11, Pages 1098: Human Dental Pulp Stem Cells Exhibit Different Biological Behaviours in Response to Commercial Bleaching Products

Materials doi: 10.3390/ma11071098

Authors: Carmen Llena Mar Collado-González Christopher Joseph Tomás-Catalá David García-Bernal Ricardo Elías Oñate-Sánchez Francisco Javier Rodríguez-Lozano Leopoldo Forner

The purpose of this study was to evaluate the diffusion capacity and the biological effects of different bleaching products on human dental pulp stem cells (hDPSCs). The bleaching gel was applied for 90, 30 or 15 min to enamel/dentine discs that adapted in an artificial chamber. The diffusion of hydrogen peroxide (HP) was analysed by fluorometry and the diffusion products were applied to hDPSCs. Cell viability, cell migration and cell morphology assays were performed using the eluates of diffusion products. Finally, cell apoptosis and the expression of mesenchymal stem cell markers were analysed by flow cytometry. Statistical analysis was performed using analysis of variance and Kruskal–Wallis or Mann–Whitney tests (α < 0.05). Significant reductions of approximately 95% in cell viability were observed for the 3 × 15 min groups (p < 0.001), while 1 × 30 min of PerfectBleach and 1 × 90 min of PolaNight resulted in reductions of 50% and 60% in cell viability, respectively (p < 0.001). Similar results were obtained in the migration assay. Moreover, the 3 × 15 min group was associated with cell morphology alterations and reductions of >70% in cell live. Finally, hDPSCs maintained their mesenchymal phenotype in all conditions. Similar concentrations of carbamide peroxide (CP) and HP in different commercial products exhibited different biological effects on hDPSCs.

Authors:   Llena, Carmen ; Collado-González, Mar ; Tomás-Catalá, Christopher Joseph; García-Bernal, David ; Oñate-Sánchez, Ricardo Elías; Rodríguez-Lozano, Francisco Javier; Forner, Leopoldo
Journal:   Materials
Volume:   11
edition:   7
Year:   2018
Pages:   1098
DOI:   10.3390/ma11071098
Publication date:   27-Jun-2018
Facts, background information, dossiers
  • fluorometry
  • flow cytometry
  • Expression
  • enamel
  • cell migration
  • apoptosis
More about Molecular Diversity Preservation International
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