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Sensors, Vol. 18, Pages 2028: Immunogold Nanoparticles for Rapid Plasmonic Detection of C. sakazakii

Sensors, Vol. 18, Pages 2028: Immunogold Nanoparticles for Rapid Plasmonic Detection of C. sakazakii

Sensors doi: 10.3390/s18072028

Authors: Mohamed A. Aly Konrad J. Domig Wolfgang Kneifel Erik Reimhult

Cronobacter sakazakii is a foodborne pathogen that can cause a rare, septicemia, life-threatening meningitis, and necrotizing enterocolitis in infants. In general, standard methods for pathogen detection rely on culture, plating, colony counting and polymerase chain reaction DNA-sequencing for identification, which are time, equipment and skill demanding. Recently, nanoparticle- and surface-based immunoassays have increasingly been explored for pathogen detection. We investigate the functionalization of gold nanoparticles optimized for irreversible and specific binding to C. sakazakii and their use for spectroscopic detection of the pathogen. We demonstrate how 40-nm gold nanoparticles grafted with a poly(ethylene glycol) brush and functionalized with polyclonal antibodies raised against C. sakazakii can be used to specifically target C. sakazakii. The strong extinction peak of the Au nanoparticle plasmon polariton resonance in the optical range is used as a label for detection of the pathogens. Individual binding of the nanoparticles to the C. sakazakii surface is also verified by transmission electron microscopy. We show that a high degree of surface functionalization with anti-C. sakazakii optimizes the detection and leads to a detection limit as low as 10 CFU/mL within 2 h using a simple cuvette-based UV-Vis spectrometric readout that has great potential for further optimization.

Authors:   Aly, Mohamed A.; Domig, Konrad J.; Kneifel, Wolfgang ; Reimhult, Erik
Journal:   Sensors
Volume:   18
edition:   7
Year:   2018
Pages:   2028
DOI:   10.3390/s18072028
Publication date:   25-Jun-2018
Facts, background information, dossiers
  • PCR
  • polyclonal antibodies
  • pathogens
  • necrotizing enterocolitis
  • meningitis
  • immunoassays
  • ethylene glycol
  • detection limit
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