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Molecules, Vol. 23, Pages 1574: A Novel HPLC Method for Direct Detection of Nitric Oxide Scavengers from Complex Plant Matrices and Its Application to Aloysia triphylla Leaves

Molecules, Vol. 23, Pages 1574: A Novel HPLC Method for Direct Detection of Nitric Oxide Scavengers from Complex Plant Matrices and Its Application to Aloysia triphylla Leaves

Molecules doi: 10.3390/molecules23071574

Authors: Didier Fraisse Alexandra Degerine-Roussel Alexis Bred Samba Fama Ndoye Magali Vivier Catherine Felgines François Senejoux

The present study aimed at developing an original pre-column HPLC assay allowing rapid characterization of nitric oxide (NO) scavengers from complex plant extracts. Sodium nitroprusside (SNP) was employed as a NO donor and spiked with an aqueous extract from Aloysia triphylla leaves prior to HPLC analysis. Relying on the ability of radical scavenging constituents to be oxidized upon reaction with radicals, this assay successfully allowed direct identification of three potential NO scavengers, including verbascoside, isoverbascoside, and luteolin-7-O-diglucuronide. These three phenolics were also individually assessed for their NO scavenging activities by using a Griess colorimetric assay. With respective IC50 values of 56 ± 4, 51 ± 3, and 69 ± 5 µg/mL, verbascoside, isoverbascoside, and luteolin-7-O-diglucuronide were all reported as potent NO scavenging compounds, confirming the efficiency of the SNP spiking HPLC assay. The present method can, thus, be considered as a valuable and effective approach for speeding up the discovery of NO scavenging constituents.

Authors:   Fraisse, Didier ; Degerine-Roussel, Alexandra ; Bred, Alexis ; Ndoye, Samba Fama; Vivier, Magali ; Felgines, Catherine ; Senejoux, François
Journal:   Molecules
Volume:   23
edition:   7
Year:   2018
Pages:   1574
DOI:   10.3390/molecules23071574
Publication date:   28-Jun-2018
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