IJMS, Vol. 19, Pages 1933: p19-Targeting ILP Protein Blockers of IL-23/Th-17 Pro-Inflammatory Axis Displayed on Engineered Bacteria of Food Origin
International Journal of Molecular Sciences doi: 10.3390/ijms19071933
Authors:
Katja Škrlec
Petra Zadravec
Marie Hlavničková
Milan Kuchař
Lucie Vaňková
Hana Petroková
Lucie Křížová
Jiří Černý
Aleš Berlec
Petr Malý
IL-23-mediated Th-17 cell activation and stimulation of IL-17-driven pro-inflammatory axis has been associated with autoimmunity disorders such as Inflammatory Bowel Disease (IBD) or Crohn’s Disease (CD). Recently we developed a unique class of IL-23-specific protein blockers, called ILP binding proteins that inhibit binding of IL-23 to its cognate cell-surface receptor (IL-23R) and exhibit immunosuppressive effect on human primary blood leukocytes ex vivo. In this study, we aimed to generate a recombinant Lactococcus lactis strain which could serve as in vivo producer/secretor of IL-23 protein blockers into the gut. To achieve this goal, we introduced ILP030, ILP317 and ILP323 cDNA sequences into expression plasmid vector containing USP45 secretion signal, FLAG sequence consensus and LysM-containing cA surface anchor (AcmA) ensuring cell-surface peptidoglycan anchoring. We demonstrate that all ILP variants are expressed in L. lactis cells, efficiently transported and secreted from the cell and displayed on the bacterial surface. The binding function of AcmA-immobilized ILP proteins is documented by interaction with a recombinant p19 protein, alpha subunit of human IL-23, which was assembled in the form of a fusion with Thioredoxin A. ILP317 variant exhibits the best binding to the human IL-23 cytokine, as demonstrated for particular L.lactis-ILP recombinant variants by Enzyme-Linked ImmunoSorbent Assay (ELISA). We conclude that novel recombinant ILP-secreting L. lactis strains were developed that might be useful for further in vivo studies of IL-23-mediated inflammation on animal model of experimentally-induced colitis.
Authors: |
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Škrlec, Katja ; Zadravec, Petra ; Hlavničková, Marie ; Kuchař, Milan ; Vaňková, Lucie ; Petroková, Hana ; Křížová, Lucie ; Černý, Jiří ; Berlec, Aleš ; Malý, Petr |
Journal: |
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International Journal of Molecular Sciences
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Volume: |
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19 |
edition: |
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7 |
Year: |
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2018 |
Pages: |
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1933 |
DOI: |
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10.3390/ijms19071933
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Publication date: |
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01-Jul-2018 |