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Human Plasma Copper Proteins Speciation by Size Exclusion Chromatography Coupled to Inductively Coupled Plasma Mass Spectrometry. Solutions for Columns Calibration by Sulfur Detection

Among the hyphenated techniques used to probe and identify metalloproteins, size exclusion chromatography coupled to inductively coupled plasma mass spectrometry (SEC-ICP-MS) has shown to have a central place. However, the calibration of SEC columns reveals to be tedious and always involves UV detection prior to ICP-MS. The presence of sulfur in 98% of proteins allows their detection by quadrupole ICP-MS, despite the isobaric interference (16O16O) on S, by monitoring 32S16O at mass to charge ratio (m/z) 48. The formation of SO occurs spontaneously in the argon plasma but can be optimized by the introduction of oxygen gas into a reaction cell (RC) to achieve nM levels. In this article, sulfur detection was discussed upon instrumental conditions and S detection was then optimized by applying O2 as a reaction gas. SO formation was used to calibrate SEC columns without UV detection. This simple SEC-ICP-MS method was used for plasma copper proteins in plasma healthy subjects (HS) and an untreated Wilson diseas...

Authors:   Souleiman El Balkhi; Joël Poupon; Jean-Marc Trocello; France Massicot; France Woimant; Olivier Laprévote
Journal:   Analytical Chemistry
Year:   2010
DOI:   10.1021/ac101128x
Publication date:   19-Jul-2010
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