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Determination of Eupatilin in Human Plasma by High-Performance Liquid Chromatography–Tandem Mass Spectrometry

A rapid, sensitive and selective liquid chromatography-tandem mass spectrometric method (LC-MS-MS) for the determination of eupatilin in human plasma has been developed. Eupatilin and an internal standard; ( S)- N-(3-{3-fluoro-4-[6-(1-methyl-1 H-tetrazol-5-yl)-pyridine-3-yl]-phenyl}-2-oxo-oxazolidin-5-ylmethyl)-acetamide (DA-7867) were extracted from human plasma by liquid-liquid extractionand analyzed on a phenyl-hexyl column using the mobile phase: acetonitrile-ammonium formate (10 mM, pH 3.0) (60:40, ν/ν). Analytes were detected using electrospray ionization-tandem mass spectrometry in multiple-reaction monitoring mode. The calibration curve was linear ( r=0.999) over the concentration range: 1.00–500 ng mL −1 with a lower limit of quantification of 1.0 ng mL −1 using a 100 μL plasma sample. The precision (CV%) of this assay ranged: 2.4–7.0%, relative error: −7.0 to +2.0%. Recoveries of eupatilin ranged: 64.3–65.0%, with that of DA-7867 (internal standard) being 87.0 ± 5.3%.

  • Content Type Journal Article
  • Pages -
  • DOI 10.1365/s10337-003-0221-0
  • Authors
    • Hye Won Lee, Drug Metabolism and Bioanalysis Laboratory College of Pharmacy and Phytofermentation Research Center, Wonkwang University Iksan 570–749 Korea
    • Hye Young Ji, Drug Metabolism and Bioanalysis Laboratory College of Pharmacy and Phytofermentation Research Center, Wonkwang University Iksan 570–749 Korea
    • Hui Hyun Kim, Drug Metabolism and Bioanalysis Laboratory College of Pharmacy and Phytofermentation Research Center, Wonkwang University Iksan 570–749 Korea
    • Il Kwang Kim, Department of Chemistry Wonkwang University Iksan 570–749 Korea
    • Soon Hoe Kim, Research Laboratories Dong-A Pharm. Co., Ltd 47-5 Sanggal-Ri Kiheung-Up, Yongin-Si, Kyunggi-Do 449-900 Korea
    • Won Bae Kim, Research Laboratories Dong-A Pharm. Co., Ltd 47-5 Sanggal-Ri Kiheung-Up, Yongin-Si, Kyunggi-Do 449-900 Korea
    • Hye Suk Lee, Drug Metabolism and Bioanalysis Laboratory College of Pharmacy and Phytofermentation Research Center, Wonkwang University Iksan 570–749 Korea
    • Journal Chromatographia
    • Online ISSN 1612-1112
    • Print ISSN 0009-5893

Authors:   Hye Won Lee, Hye Young Ji, Hui Hyun Kim, Il Kwang Kim, Soon Hoe Kim, Won Bae Kim, Hye Suk Lee
Journal:   Chromatographia
Year:   2004
DOI:   10.1365/s10337-003-0221-0
Publication date:   30-Mar-2004
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