A high performance liquid chromatographic method has been developed for the simultaneous determination of hypoxanthine (Hx), xanthine (Xa), guanine (Gu) and adenine (Ad) in shellfish. The separation of these compounds was performed on a porous graphitic carbon column (100 × 4.6 mm I.D.) using acetonitrile-phosphate buffer (23/77 v/v) containing 0.5% trichloroacetic acid at pH 2.0 as the mobile phase. The flow rate was fixed to 1 mLmin −1 and the detection was monitored at 254 nm. Calibration curves were found to be linear in the concentration range from 0.3 to 100 μgmL −1 with correlation coefficients ( r 2) > 0.999. The lowest detectable concentrations of Hx, Xa, Gu and Ad were 0.04, 0.06, 0.08, 0.07 μg mL −1 respectively. Recovery of all purine standards added to sea urchin gonad was above 97%. The proposed method was successfully applied for the determination of Hx, Xa, Gu and Ad in sea urchin gonad extract.
- Content Type Journal Article
- Pages -
- DOI 10.1365/s10337-003-0223-y
- L. Monser, Institut National des Sciences Appliquées et de Technologie Centre Urbain Nord B.P.N°676 1080 Tunis Cedex Tunisia
- Journal Chromatographia
- Online ISSN 1612-1112
- Print ISSN 0009-5893