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Low angle light scattering analysis: a novel quantitative method for functional characterization of human and murine platelet receptors

Abstract Background: Determinations of platelet receptor functions are indispensable diagnostic indicators of cardiovascular and hemostatic diseases including hereditary and acquired receptor defects and receptor responses to drugs. However, presently available techniques for assessing platelet function have some disadvantages, such as low sensitivity and the requirement of large sample sizes and unphysiologically high agonist concentrations. Our goal was to develop and initially characterize a new technique designed to quantitatively analyze platelet receptor activation and platelet function on the basis of measuring changes in low angle light scattering. Methods: We developed a novel technique based on low angle light scattering registering changes in light scattering at a range of different angles in platelet suspensions during activation. Results: The method proved to be highly sensitive for simultaneous real time detection of changes in size and shape of platelets during activation. Unlike commonly-u...

Authors:   Igor Mindukshev; Stepan Gambaryan; Linda Kehrer; Claudia Schuetz; Anna Kobsar; Natalia Rukoyatkina; Viacheslav O. Nikolaev; Alexander Krivchenko; Steve P. Watson; Ulrich Walter; Joerg Geiger
Journal:   Clinical Chemistry
Year:   2011
DOI:   10.1515/CCLM.2011.817
Publication date:   13-Dec-2011
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