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Rapid Determination of Chlormequat in Meat by Dispersive Solid-Phase Extraction and Hydrophilic Interaction Liquid Chromatography (HILIC)–Electrospray Tandem Mass Spectrometry

A rapid method for analyzing trace levels of chlormequat (CQ) in meat samples by hydrophilic interaction liquid chromatography (HILIC)–electrospray tandem mass spectrometry was developed. The samples were extracted with acetonitrile, followed by a rapid cleanup through a dispersive solid-phase extraction (DSPE) technique with octadecyl (C18) DSPE sorbents. The chromatographic separation was achieved within 6 min using a HILIC column with 10 mM ammonium acetate and 0.1% (v/v) formic acid in water/acetonitrile (v/v, 40:60) as the mobile phase. Quantification was performed using a matrix-matched calibration curve, which was linear in the range of the 0.05–100 μg/L. The limit of detection (LOD) was estimated at 0.03 μg/kg for CQ on the basis of a peak to peak signal noise (S/N = 3). The limit of quantification (LOQ) was 0.1 μg/kg on the basis of the lowest spiked concentration with suitable precision and accuracy. The average recovery of CQ in spiked meat samples was 86.4–94.7% at 2, 20, and 200 μg/kg. Finall...

Authors:   Chunmei Li; Fen Jin; Zhiyong Yu; Yamei Qi; Xiaomei Shi; Miao Wang; Hua Shao; Maojun Jin; Jing Wang; Mingqi Yang
Journal:   Journal of Agricultural and Food Chemistry
Year:   2012
DOI:   10.1021/jf3010756
Publication date:   25-Jun-2012
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