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Electrowetting‐on‐Dielectric actuation of droplets with capillary electrophoretic zones for MALDI mass spectrometric analysis

An automated fraction collection interface was developed for coupling capillary electrophoresis (CE) with matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS). This fraction collection approach is based on the electrowetting on dielectric phenomenon (EWOD) performed on a digital microfluidic (DMF) board; it does not rely on a MALDI spotter. In this study, a four‐peptide mixture was used as a sample test, and the separations were conducted in a portable CE instrument with a 150 μm o.d. x 50 μm i.d. capillary and a contactless conductivity detector (CCD). The CE instrument was interfaced with a robust DMF board. The CE fractions were directly deposited onto the DMF board at predetermined locations prior to MALDI analysis. The series of experiments determined the lowest concentration that produces a measurable MALDI signal. The concentrations were 0.25, 0.5, 0.05, and 0.05 nmol for Bradykinin, Angiotensin, ACTH and insulin, respectively. The CCD limit of detection for the same analytes was 2.5 μmol.

Authors:   Jelena Gorbatsova, Maria Borissova, Mihkel Kaljurand
Year:   2012
Pages:   n/a
DOI:   10.1002/elps.201200096
Publication date:   04-Jul-2012
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