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pH-dependent self-association of zinc-free insulin characterized by concentration-gradient static light scattering

Insulin self-association at pH 1.85, 1.95, 3.0, 7.2, 8.0 and 10 was studied via composition gradient light scattering (CG-SLS). At pH 1.95 in acetic acid, insulin was found to exist as a monomer, and in pH 1.85 HCl as a dimer. At pH values of 3.0 – 8.0, the dependence of scattering intensity upon total insulin concentration at concentrations of up to 1.5mg/mL may be quantitatively accounted for by a simple isodesmic association equilibrium scheme requiring only a single association constant for addition of monomer to monomer or any oligomer. At pH 10, the association constant for addition of monomer...

Authors:   Arun K., Attri , Cristina, Fernández , Allen P., Minton
Journal:   Biophysical Chemistry
Year:   2010
DOI:   10.1016/j.bpc.2010.02.002
Publication date:   08-Feb-2010
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