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One-Step Sensitive Detection of Salmonella typhimurium by Coupling Magnetic Capture and Fluorescence Identification with Functional Nanospheres

Sensitive, rapid, and reliable detection of bacteria has always been pursued due to the great threat of the bacteria to human health. In this study, a convenient one-step strategy for detecting Salmonella typhimurium was developed. Immunomagnetic nanospheres (IMNS) and immunofluorescent nanospheres (IFNS) were used to specifically capture and recognize S. typhimurium simultaneously. After magnetic separation, the sandwich immune complexes (IMNS–bacteria–IFNS) were detected under a fluorescence microscope with a detection limit as low as ca. 10 CFU/mL. When they were detected by fluorescence spectrometer, a linear range was exhibited at the concentration from 105 to 107 CFU/mL with R2 = 0.9994. Compared with the two-step detection strategy, in which the bacteria were first captured with the IMNS and subsequently identified with the IFNS, this one-step strategy simplified the detection process and improved the sensitivity. Escherichia coli and Shigella flexneri both showed negative results with this method,...

Authors:   Cong-Ying Wen; Jun Hu; Zhi-Ling Zhang; Zhi-Quan Tian; Guo-Ping Ou; Ya-Long Liao; Yong Li; Min Xie; Zi-Yong Sun; Dai-Wen Pang
Journal:   Analytical Chemistry
Year:   2013
DOI:   10.1021/ac303204q
Publication date:   04-Jan-2013
Facts, background information, dossiers
  • bacteria
  • Salmonella typhimurium
  • magnetic separators
  • Escherichia coli
  • couplings
  • concentration
More about American Chemical Society Publications
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