My watch list
my.chemeurope.com  
Login  

NIR- and FRET-Based Sensing of Cu2+ and S2- in Physiological Conditions and in Live Cells

We have synthesized a new indole functionalized rhodamine derivative L1 which specifically binds to Cu2+ in the presence of large excess of other competing ions with visually observable changes in their electronic and fluorescence spectral behavior. These spectral changes are significant enough in the NIR and visible region of the spectrum and thus enable naked eye detection. The receptor, L1, could be employed as a resonance energy transfer (RET) based sensor for detection of Cu2+ based on the process involving the donor indole and the acceptor Cu2+ bound xanthene fragment. Studies reveal that L1–Cu complex is selectively and fully reversible in presence of sulfide anions. Further, fluorescence microscopic studies confirmed that the reagent L1 could also be used as an imaging probe for detection of uptake of these ions in HeLa cells.

Authors:   Chirantan Kar; Manab Deb Adhikari; Aiyagari Ramesh; Gopal Das
Journal:   Inorganic Chemistry
Year:   2013
DOI:   10.1021/ic301872q
Publication date:   09-Jan-2013
Facts, background information, dossiers
More about American Chemical Society Publications
Your browser is not current. Microsoft Internet Explorer 6.0 does not support some functions on Chemie.DE