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Identification of Metal‐Associated Proteins in Cells by Using Continuous‐Flow Gel Electrophoresis and Inductively Coupled Plasma Mass Spectrometry

Metal detector: The title strategy provides a new way for rapid profiling of the metal‐associated proteins on a proteome‐ and metallome‐wide scale. The technique was applied to identify protein targets of a bismuth‐based drug in Helicobacter pylori and to study the metal binding of the protein SlyD, which is a member of the FK506‐binding protein family, in E. coli.

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Authors:   Ligang Hu, Tianfan Cheng, Bin He, Lu Li, Yuchuan Wang, Yau‐Tsz Lai, Guibin Jiang, Hongzhe Sun
Journal:   Angewandte Chemie International Edition
Year:   2013
Pages:   n/a
DOI:   10.1002/anie.201300794
Publication date:   02-Apr-2013
Facts, background information, dossiers
  • Helicobacter pylori
  • gel electrophoresis
  • cells
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