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Evaluation of matrix effects in different multi‐pesticide residue analysis methods using liquid chromatography – tandem mass spectrometry, including an automated two‐dimensional clean‐up approach

Pesticide residue analysis is an important part of food quality control. Three of the most widely used methods are the DFG S19 (extraction with acetone), the ChemElut method (extraction with methanol) and QuEChERS (acetonitrile‐based). Despite many developments in the field of sample preparation, matrix effects are still one of the most disturbing problems in routine analysis. In this study we compare the matrix effects in liquid chromatography – mass spectrometry analysis after use of these three methods in pesticide analysis. Using postcolumn infusion, we were able to visualize all suppressions over the whole chromatogram in matrix effect profiles. Recently, we also presented a system for the determination of up to 300 pesticides from various kinds of fruit and vegetables. For the measurement, we injected an aliquot of a raw acetonitrile extract. The subsequent clean‐up was carried out fully automated by a multi‐dimensional liquid chromatography. Matrix compounds and analytes are separated in the first dimension on a HILIC column. In this study we also compared this new approach with the classical methods. The matrix effect profiles showed less suppression with the HILIC‐based clean‐up. A final evaluation of the partitioning steps of all methods confirmed the benefits of the chromatographic clean‐up approach.

This article is protected by copyright. All rights reserved

Authors:   Stefan Kittlaus, Günther Kempe, Karl Speer
Journal:   Journal of Separation Science
Year:   2013
Pages:   n/a
DOI:   10.1002/jssc.201300044
Publication date:   07-May-2013
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