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Ultra‐high‐performance size‐exclusion chromatography of synthetic polymers. 1. Demonstration of capability

Ultra‐high‐performance size‐exclusion chromatography (UHP SEC) is a newly developed disruptive technology that allows the high‐resolution separation of synthetic polymers in as little as two minutes. The capability of UHP SEC for the characterization of synthetic polymers in both organic solvents and aqueous buffers has been demonstrated. Using the Waters ACQUITY® UPLC®1 H‐Class system and ethylene‐bridged hybrid (BEH) SEC columns packed with 1.7–2.5 μm particles with pore sizes ranging from 45 to 900 Å, size‐based separations of polystyrene and poly(methyl methacrylate) standards in tetrahydrofuran and poly(ethylene oxide) standards in 20 mM ammonium acetate in methanol are achieved within two to four minutes. The speed of analysis is about ten times faster than conventional SEC separations, and greater resolution is achieved. Average molecular weights of selected commercial polymers have been determined using UHP and conventional SEC. Average M data of analyzed samples are in good agreement using the two approaches. An inherent limitation of SEC in UHP mode is the characterization of very high M polymers (above ca. 2 million Da) due to the deformation and/or mechanical shearing of large molecules at high flow rates.

This article is protected by copyright. All rights reserved

Authors:   Miroslav Janco, James N. Alexander, Edouard S. P. Bouvier, Damian Morrison
Journal:   Journal of Separation Science
Year:   2013
Pages:   n/a
DOI:   10.1002/jssc.201300444
Publication date:   02-Jul-2013
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