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Binding of Doxyl Stearic Spin Labels to Human Serum Albumin: An EPR Study

The binding of spin-labeled fatty acids (SLFAs) to the human serum albumin (HSA) examined by electron paramagnetic resonance (EPR) spectroscopy was studied to evaluate the potential of the HSA/SLFA/EPR technique as a biomarking tool for cancer. A comparative study was performed on two spin labels with nitroxide groups attached at opposite ends of the fatty acid (FA) chain, 5-doxyl stearic (5-DS) and 16-doxyl stearic (16-DS) acid. The effects of incubation time, different [SLFA]/[HSA] molar ratios, ethanol, and temperature showed that the position of the nitroxide group produces certain differences in binding between the two SLFAs. Spectra for different [SLFA]/[HSA] molar ratios were decomposed into two spectral components, which correspond to the weakly and strongly bound SLFAs. The reduction of SLFA with ascorbate showed the existence of a two component process, fast and slow, confirming the decomposition results. Warfarin has no effect on the binding of the two SLFAs, whereas ibuprofen significantly dec...

Authors:   Aleksandra A. Pavićević; Ana D. Popović-Bijelić; Miloš D. Mojović; Snežana V. Šušnjar; Goran G. Bačić
Journal:   Journal of Physical Chemistry B
Year:   2014
DOI:   10.1021/jp5068928
Publication date:   09-Sep-2014
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