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Intrinsic biocontainment: Multiplex genome safeguards combine transcriptional and recombinational control of essential yeast genes [Genetics]

Biocontainment may be required in a wide variety of situations such as work with pathogens, field release applications of engineered organisms, and protection of intellectual properties. Here, we describe the control of growth of the brewer’s yeast, Saccharomyces cerevisiae, using both transcriptional and recombinational “safeguard” control of essential gene function....

Authors:   Yizhi Cai; Neta Agmon; Woo Jin Choi; Alba Ubide; Giovanni Stracquadanio; Katrina Caravelli; Haiping Hao; Joel S. Bader; Jef D. Boeke
Journal:   Proceedings of the National Academy of Sciences current issue
Volume:   112
edition:   6
Year:   2015
Pages:   1803
DOI:   10.1073/pnas.1424704112
Publication date:   10-Feb-2015
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