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Pillar Cuvettes: Capillary-Filled, Microliter Quartz Cuvettes with Microscale Path Lengths for Optical Spectroscopy

The goal of most analytical techniques is to reduce the lower limit of detection; however, it is sometimes necessary to do the opposite. High sample concentrations or samples with high molar absorptivity (e.g., dyes and metal complexes) often require multiple dilution steps or laborious sample preparation prior to spectroscopic analysis. Here, we demonstrate dilution-free, one-step UV–vis spectroscopic analysis of high concentrations of platinum(IV) hexachloride in a micropillar array, that is, “pillar cuvette”. The cuvette is spontaneously filled by wicking of the liquid sample into the micropillar array. The pillar height (thus, the film thickness) defines the optical path length, which was reduced to between 10 and 20 μm in this study (3 orders of magnitude smaller than in a typical cuvette). Only one small droplet (∼2 μL) of sample is required, and the dispensed volume need not be precise or even known to the analyst for accurate spectroscopy measurements. For opaque pillars, we show that absorbance i...

Authors:   Gregor Holzner; Frederik Hermanus Kriel; Craig Priest
Journal:   Analytical Chemistry
Year:   2015
DOI:   10.1021/acs.analchem.5b00860
Publication date:   20-Apr-2015
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