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Electronic Olfactory Sensor Based on A. mellifera Odorant‐Binding Protein 14 on a Reduced Graphene Oxide Field‐Effect Transistor

Abstract

An olfactory biosensor based on a reduced graphene oxide (rGO) field‐effect transistor (FET), functionalized by the odorant‐binding protein 14 (OBP14) from the honey bee (Apis mellifera) has been designed for the in situ and real‐time monitoring of a broad spectrum of odorants in aqueous solutions known to be attractants for bees. The electrical measurements of the binding of all tested odorants are shown to follow the Langmuir model for ligand–receptor interactions. The results demonstrate that OBP14 is able to bind odorants even after immobilization on rGO and can discriminate between ligands binding within a range of dissociation constants from Kd=4 μM to Kd=3.3 mM. The strongest ligands, such as homovanillic acid, eugenol, and methyl vanillate all contain a hydroxy group which is apparently important for the strong interaction with the protein.

Ein Sensor auf Graphenbasis, der den Geruchssinn der Honigbiene nachahmt: Elektrische Messungen zeigen die Bindung anziehender Duftstoffe an das immobilisierte, duftstoffbindende Protein 14 (OBP14) an. Der Sensor unterscheidet in Echtzeit und quantitativ zwischen Duftstoffen, und er liefert vollständige Reaktionskinetiken der Ligand‐Rezeptor‐Wechselwirkungen. Dabei zeigt sich die Bedeutung eines Hydroxysubstituenten für die Erkennung aromatischer Duftstoffe.

Authors:   Melanie Larisika, Caroline Kotlowski, Christoph Steininger, Rosa Mastrogiacomo, Paolo Pelosi, Stefan Schütz, Serban F. Peteu, Christoph Kleber, Ciril Reiner‐Rozman, Christoph Nowak, Wolfgang Knoll
Journal:   Angewandte Chemie
Year:   2015
Pages:   n/a
DOI:   10.1002/ange.201505712
Publication date:   14-Sep-2015
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