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Direct Interface between Digital Microfluidics and High Performance Liquid Chromatography–Mass Spectrometry

We introduce an automated method to facilitate in-line coupling of digital microfluidics (DMF) with HPLC-MS, using a custom, 3D-printed manifold and a custom plugin to the popular open-source control system, DropBot. The method was designed to interface directly with commercial autosamplers (with no prior modification), suggesting that it will be widely accessible for end-users. The system was demonstrated to be compatible with samples dissolved in aqueous buffers and neat methanol and was validated by application to a common steroid-labeling derivatization reaction. We propose that the methods described here will be useful for a wide range of applications, combining the automated sample processing power of DMF with the resolving and analytical capacity of HPLC-MS.

Authors:   Chang Liu; Kihwan Choi; Yang Kang; Jihye Kim; Christian Fobel; Brendon Seale; J. Larry Campbell; Thomas R. Covey; Aaron R. Wheeler
Journal:   Analytical Chemistry
Year:   2015
DOI:   10.1021/acs.analchem.5b03616
Publication date:   23-Nov-2015
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