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Probing Propeller-Like Loops of DNA G-quadruplexes with Looped-Out 2-Aminopurine for Label-Free Switchable Molecular Sensing

26-Jun-2018 | Pai Peng; Yi Du; Yudie Sun; Shuangna Liu; Lan Mi; Tao Li, Analyst, 2018

We report a new signal readout mechanism for DNA molecular sensing devices using ligand-free fluorogenic G-quadruplexes of which the propeller-like loops are distinguished from the diagonal and lateral ones with incorporated 2-aminopurine (2-AP, a fluorescent analogue of adenine). We study the ...

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Optimised quadrupole mass spectrometer with a dual filter analyser for in-field chemical sniffing of volatile organic compounds

26-Jun-2018 | Boris Brkic; Neil France; Stamatios Giannoukos; Stephen Taylor, Analyst, 2018

We report a novel portable 17 kg system based on a quadrupole mass spectrometer (QMS) with electronics power consumption of 24 W. The system can be used for in-field identification of gases and volatile/semivolatile organic compounds (VOCs/SVOCs). The mass analyser is a custom-made quadrupole ...

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Cathodic electrochemiluminescence behaviour of MoS2 quantum dots and its biosensing for microRNA-21

26-Jun-2018 | Juntao Cao; Fu-Rao Liu; Fang Hou; Juan Peng; Shu-Wei Ren; Yan-Ming Liu, Analyst, 2018

The cathodic electrochemiluminescence (ECL) behaviour of nontoxic MoS2 quantum dots (QDs) was firstly studied using potassium peroxydisulfate as co-reactant. Ag-PAMAM NCs serving as difunctional tags for quenching and enhancing ECL of MoS2-reduced graphene oxide composites were introduced into ...

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A novel, fast and sensitive supercritical fluid chromatography-tandem mass spectrometry (SFC-MS/MS) method for analysis of arachidonic acid metabolites

26-Jun-2018 | Jonas Bergquist; Kumari Ubhayasekera; Santosh Raman Acharya, Analyst, 2018

The development of a rapid, sensitive and reliable method for the quantification of bioactive arachidonic acid metabolites (AA-metabolites) in biological samples is quite challenging due to the minute concentrations, short half-life and their structural complexity arising from different isomers. ...

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Spatially encoded diffusion-ordered NMR spectroscopy of reaction mixtures in organic solvents

26-Jun-2018 | Ghanem Hamdoun; Ludmilla Guduff; Carine van Heijenoort; Christophe Bour; Vincent Gandon; Jean-Nicolas Dumez, Analyst, 2018

Diffusion-ordered NMR spectroscopy (DOSY) is a powerful method for the analysis of mixtures. Classic DOSY methods require several minutes of acquisition, and we show here that DOSY experiments can be recorded in less than one second for the challenging case of solution mixtures in low-viscosity ...

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Thermosensitive molecularly imprinted core-shell CdTe quantum dots as a ratiometric fluorescence nanosensor for phycocyanin recognition and detection in seawater

26-Jun-2018 | Jinhua Li; Junqing Fu; Qian Yang; Liyan Wang; Xiaoyan Wang; Lingxin Chen, Analyst, 2018

Versatile molecular imprinting fluorescence sensors have been prepared for various species, but imprinting based fluorescent detection of target proteins upon different external stimuli is rarely reported. Herein, a novel phycocyanin-imprinted ratiometric fluorescence nanosensor was developed for ...

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A Robust Split-Luciferase-Based Cell Fusion Screening for Discovering Myogenesis-Promoting Molecules

26-Jun-2018 | Qiaojing Li; Hideaki Yoshimura; Maki Komiya; Ken Tajiri; Motonari Uesugi; Yutaka Hata; Takeaki Ozawa, Analyst, 2018

Myogenesis-promoting chemicals are an important source of new pharmaceuticals for the treatment of skeletal muscle atrophy that impairs quality of life. This report presents a robust and quantitative bioluminescence-based assay for screening myogenesis-promoting compounds in chemical libraries. ...

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Near-infrared incoherent broadband cavity enhanced absorption spectroscopy (NIR-IBBCEAS) for detection and quantification of natural gas components

26-Jun-2018 | Neeraj Prakash; Arun Ramachandran; Ravi Varma; Jun Chen; Claudio Mazzoleni; Ke Du, Analyst, 2018

The principle of near-infrared incoherent broadband cavity enhanced absorption spectroscopy was employed to develop a novel instrument for detecting natural gas leaks as well as for testing the quality of natural gas mixtures. The instrument utilizes the absorption features of methane, butane, ...

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Direct-Laser-Writing of Three-Dimensional Porous Graphene Frameworks on Indium-Tin Oxide for Sensitive Electrochemical Biosensing

20-Jun-2018 | Qing Hong; Limin Yang; Lei Ge; Zhenhui Liu; Feng Li, Analyst, 2018

The fabrication of graphene electrode with three-dimensional (3D) porous architecture would be highly desirable for electrochemical (bio-)sensing. Direct-laser-writing (DLW) on polyimide sheet has been recognized as an advance approach to pattern 3D porous graphene frameworks (3DPGFs)-based ...

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Integrated strategy for high-sensitive phosphoproteome analysis from low micrograms of protein samples

20-Jun-2018 | Wendong Chen; Lan Chen; Ruijun Tian, Analyst, 2018

Phosphoproteomics has become popular proteomic technology for exploring cellular signaling networks. However, current approaches often require milligrams of protein samples which hamper their applications for translational purpose with limited starting materials. In this study, we aimed to ...

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