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1,115 Newest Publications in csh protocols

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Fluorescence In Situ Hybridization for the Identification of Environmental Microbes

01-Jan-2010 | Wendeberg, Annelie, CSH Protocols, 2010

INTRODUCTION This protocol presents methods for the phylogenetic identification of microorganisms in environmental samples (e.g., water and sediments) by means of fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes, followed by signal amplification with catalyzed ...

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Preparation and Culture of Limb Blastema Stem Cells from Regenerating Larval and Adult Salamanders

01-Jan-2010 | Kumar, A., Godwin, J. W., CSH Protocols, 2010

INTRODUCTION Adult salamanders show extensive ability to regenerate their body parts, and this can be attributed at least in part to their cellular plasticity. Cell reprogramming, dedifferentiation, proliferation and redifferentiation occur naturally in adult salamanders in response to tissue ...

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Using the Metagenomics RAST Server (MG-RAST) for Analyzing Shotgun Metagenomes

01-Jan-2010 | Glass, E. M., Wilkening, J., Wilke, A., Antonopoulos, D., Meyer, F., CSH Protocols, 2010

INTRODUCTION Shotgun metagenomics creates millions of fragments of short DNA reads, which are meaningless unless analyzed appropriately. The Metagenomics RAST server (MG-RAST) is a web-based, open source system that offers a unique suite of tools for analyzing these data sets. After ...

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Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN-{gamma} Secreting T Cells

01-Jan-2010 | Boulet, Salix; Ndongala, Michel L.; Bernard, Nicole F., CSH Protocols, 2010

INTRODUCTION The enzyme-linked immunospot (ELISPOT) assay measures the secretion intensity of effector molecules released by immune cells in response to ex vivo antigenic stimulation, as well as the frequency of these responding cells. This assay is highly sensitive, quantitative, easy to use, ...

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Cryosectioning and Immunocytochemistry of Adult Drosophila Eye Sections

01-Jan-2010 | Wolff, T., CSH Protocols, 2010

INTRODUCTION This protocol is recommended for antibody staining of frozen adult Drosophila eye sections. It describes the tissue preparation and staining with primary and secondary antibodies.

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