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1,115 Newest Publications in csh protocolsrss
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01-Jun-2018 | Kumar, P., Nagarajan, A., Uchil, P. D., CSH Protocols, 2018
Among viability assays that depend on the conversion of substrate to chromogenic product by live cells, the MTT assay is still among one of the most versatile and popular assays. The MTT assay involves the conversion of the water-soluble yellow dye MTT ...
01-Jun-2018 | Field, C. M., Mitchison, T. J., CSH Protocols, 2018
Here, we provide methods for assembly of mitotic spindles and interphase asters in Xenopus laevis egg extract, and compare them to spindles and asters in the egg and zygote. Classic "cycled" spindles are made by adding sperm nuclei to metaphase-arrested cytostatic factor (CSF) extract and ...
01-Jun-2018 | Fishman, J. B., Berg, E. A., CSH Protocols, 2018
Caprylic acid has been used to enrich IgG from serum, ascites, and cell culture supernatants by precipitating the non-IgG serum proteins. By precipitating all of the unwanted serum proteins rather than the antibodies, the tendency of antibodies to aggregate when precipitated is avoided. This ...
01-May-2018 | Kim, T. H., Dekker, J., CSH Protocols, 2018
Owing to its digital nature, ChIP-seq has become the standard method for genome-wide ChIP analysis. Using next-generation sequencing platforms (notably the Illumina Genome Analyzer), millions of short sequence reads can be obtained. The densities of recovered ChIP sequence reads along the genome ...
01-May-2018 | Michael R. Green; Joseph Sambrook, CSH Protocols, 2018
This protocol describes the reagents and procedures required to amplify a segment of double-stranded DNA in a chain reaction catalyzed by a thermostable DNA polymerase. It is the foundation for all subsequent variations of the polymerase chain reaction (PCR).
01-May-2018 | Green, M. R., Sambrook, J., CSH Protocols, 2018
This protocol describes a variation of a simple method for isolation of DNA from mouse tails that does not require extraction with phenol:chloroform. This variant is useful when processing very large numbers of samples.
01-May-2018 | Neil, C. R., Mowry, K., CSH Protocols, 2018
Xenopus laevis oocytes are widely used to study mechanisms of RNA function and biogenesis. While the large size of Xenopus oocytes is amenable to both biochemical and imaging approaches, the relative opacity of the yolk-rich cytoplasm has limited high-resolution imaging of endogenous RNAs. Here, ...
01-May-2018 | Greenfield, E. A., DeCaprio, J., Brahmandam, M., CSH Protocols, 2018
Many compounds on their own do not have all of the properties needed to induce a strong antibody response. However, small changes in the structure of an antigen can often greatly alter the immunogenicity of a compound. Common methods for doing so include the addition of small modifying groups ...
01-May-2018 | Mochida, K., CSH Protocols, 2018
In conventional vitrification methods, the embryos are vitrified under considerable supercooling (i.e., under nonequilibrium conditions). This protocol is a refinement of newer equilibrium vitrification methods. The equilibrium vitrification solution contains a high concentration of ...