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776 Newest Publications in journal of microscopy

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An orientation‐independent DIC microscope allows high resolution imaging of epithelial cell migration and wound healing in a cnidarian model

18-Jan-2018 | J.E. MALAMY, M. SHRIBAK, Journal of Microscopy, 2018

Summary Epithelial cell dynamics can be difficult to study in intact animals or tissues. Here we use the medusa form of the hydrozoan Clytia hemisphaerica, which is covered with a monolayer of epithelial cells, to test the efficacy of an orientation‐independent differential interference ...

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An orientation‐independent DIC microscope allows high resolution imaging of epithelial cell migration and wound healing in a cnidarian model

18-Jan-2018 | J.E. MALAMY, M. SHRIBAK, Journal of Microscopy, 2018

Summary Epithelial cell dynamics can be difficult to study in intact animals or tissues. Here we use the medusa form of the hydrozoan Clytia hemisphaerica, which is covered with a monolayer of epithelial cells, to test the efficacy of an orientation‐independent differential interference ...

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A multimodal microcharacterisation of trace‐element zonation and crystallographic orientation in natural cassiterite by combining cathodoluminescence, EBSD, EPMA and contribution of confocal Raman‐in‐SEM imaging

16-Jan-2018 | G. WILLE, C. LEROUGE, U. SCHMIDT, Journal of Microscopy, 2018

Summary In cassiterite, tin is associated with metals (titanium, niobium, tantalum, indium, tungsten, iron, manganese, mercury). Knowledge of mineral chemistry and trace‐element distribution is essential for: the understanding of ore formation, the exploration phase, the feasibility of ore ...

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Progress towards a methodology for high throughput 3D reconstruction of soot nanoparticles via electron tomography

16-Jan-2018 | E. HAFFNER‐STATON, A. LA ROCCA, M.W. FAY, Journal of Microscopy, 2018

Summary The aim of this work is to make progress towards the development of 3D reconstruction as a legitimate alternative to traditional 2D characterization of soot. Time constraints are the greatest opposition to its implementation, as currently reconstruction of a single soot particle takes ...

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Oolong tea and LR‐White resin: a new method of plant sample preparation for transmission electron microscopy

15-Jan-2018 | XIAOHUA HE, FUZHEN GUO, BIN LIU, Journal of Microscopy, 2018

Summary Simplifying sample processing, shortening the sample preparation time, and adjusting procedures to suitable for new health and safety regulations, these issues are the current challenges which electron microscopic examinations need to face. In order to resolve these problems, new plant ...

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A guide to analysis and reconstruction of serial block face scanning electron microscopy data

15-Jan-2018 | E. COCKS, M. TAGGART, F.C. RIND, K. WHITE, Journal of Microscopy, 2018

Summary Serial block face scanning electron microscopy (SBF‐SEM) is a relatively new technique that allows the acquisition of serially sectioned, imaged and digitally aligned ultrastructural data. There is a wealth of information that can be obtained from the resulting image stacks but this ...

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Analogous on‐axis interference topographic phase microscopy (AOITPM)

11-Jan-2018 | P. XIU, Q. LIU, X. ZHOU, Y. XU, C. KUANG, X. LIU, Journal of Microscopy, 2018

Summary The refractive index (RI) of a sample as an endogenous contrast agent plays an important role in transparent live cell imaging. In tomographic phase microscopy (TPM), 3D quantitative RI maps can be reconstructed based on the measured projections of the RI in multiple directions. The ...

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Measurement of two‐photon‐absorption spectra through nonlinear fluorescence produced by a line‐shaped excitation beam

11-Jan-2018 | E. HASANI, J. PARRAVICINI, L. TARTARA, A. TOMASELLI, D. TOMASSINI, Journal of Microscopy, 2018

Summary We propose an innovative experimental approach to estimate the two‐photon absorption (TPA) spectrum of a fluorescent material. Our method develops the standard indirect fluorescence‐based method for the TPA measurement by employing a line‐shaped excitation beam, generating a ...

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On the influence of lipid‐induced optical anisotropy for the bioimaging of exo‐ or endocytosis with interference microscopic imaging

11-Jan-2018 | D. MARQUES, A. MIRANDA, A.G. SILVA, P.R.T. MUNRO, P.A.A. DE BEULE, Journal of Microscopy, 2018

Summary Some implementations of interference microscopy imaging use digital holographic measurements of complex scattered fields to reconstruct three‐dimensional refractive index maps of weakly scattering, semi‐transparent objects, frequently encountered in biological investigations. ...

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An optical configuration for fastidious STEM detector calibration and the effect of the objective‐lens pre‐field

08-Jan-2018 | L. JONES, A. VARAMBHIA, H. SAWADA, P.D. NELLIST, Journal of Microscopy, 2018

Summary In the scanning transmission electron microscope, an accurate knowledge of detector collection angles is paramount in order to quantify signals on an absolute scale. Here we present an optical configuration designed for the accurate measurement of collection angles for both ...

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