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451 Newest Publications of BioMed Central

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Chemoenzymatic Kinetic resolution of (R)-malathion in aqueous media

09-Sep-2015 | Enríquez-Núñez, Carlos A; Camacho-Dávila, Alejandro A; Ramos-Sánchez, Víctor H; Zaragoza-Galán, Gerardo; Ballinas-Ca ..., Chemistry Central Journal , 2015

Background: Malathion (R,S)-diethyl-2-[(dimethoxyphosphorothioyl)sulfanyl]butanedioate is a chiral organophosphorus compound used widely as pesticide for suppression of harmful insects such as mosquitoes. It is well known that in biological systems (R)-malathion is the active enantiomer, ...

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Complex kinetics and residual structure in the thermal unfolding of yeast triosephosphate isomerase

03-Sep-2015 | Labastida-Polito, Ariana; Garza-Ramos, Georgina; Camarillo-Cadena, Menandro; Zubillaga, Rafael A; Hernández-Arana, A ..., BMC Biochemistry, 2015

Background: Saccharomyces cerevisiae triosephosphate isomerase (yTIM) is a dimeric protein that shows noncoincident unfolding and refolding transitions (hysteresis) in temperature scans, a phenomenon indicative of the slow forward and backward reactions of the native-unfolded process. Thermal ...

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Purification and characterization of a cytochrome c with novel caspase-3 activation activity from the pathogenic fungus Rhizopus arrhizus

03-Sep-2015 | Saxena, Manoj; Sharma, Rohit K; Ramirez-Paz, Josell; Tinoco, Arthur D; Griebenow, Kai, BMC Biochemistry, 2015

Background: Members of Rhizopus species are the most common cause of mucormycosis, a rare but often fatal fungal infection. Host induced pathogen apoptosis and pathogen induced host cell apoptosis are often involved in fungal infections. In many organisms, the release of mitochondrial cytochrome ...

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A solid-phase extraction method using Transcarpathian clinoptilolite for preconcentration of trace amounts of terbium in water samples

27-Aug-2015 | Vasylechko, Volodymyr O; Gryshchouk, Galyna V; Zakordonskiy, Victor P; Vyviurska, Olga; Pashuk, Andriy V, Chemistry Central Journal , 2015

Background: In spite of the fact that terbium is one of the rarest elements in the Earth’s crust, it is frequently used for the production of high technological materials. At the result, an effective combination of sample preparation procedure and detection method for terbium ions in different ...

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Synthesis and biological evaluation of new nanosized aromatic polyamides containing amido- and sulfonamidopyrimidines pendant structures

19-Aug-2015 | Hassan, Hammed H A M; Mansour, Elsayed M E; Abou Zeid, Asmaa M S; El-Helow, Ehab R; Elhusseiny, Amel F; Soliman, Raafat, Chemistry Central Journal , 2015

Background: Antibiotics are biocides or products that inhibit the growth of microorganisms in the living cells and there are extensive works directed to develop efficient antimicrobial agents. The sulfonamide-containing polymers have great potential to resist gram-positive or gram-negative ...

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Identification of inhibitors that target dual-specificity phosphatase 5 provide new insights into the binding requirements for the two phosphate pockets

19-Aug-2015 | Neumann, Terrence S; Span, Elise A; Kalous, Kelsey S; Bongard, Robert; Gastonguay, Adam; Lepley, Michael A; Kutty, R ..., BMC Biochemistry, 2015

Background: Dual-specificity phosphatase-5 (DUSP5) plays a central role in vascular development and disease. We present a p-nitrophenol phosphate (pNPP) based enzymatic assay to screen for inhibitors of the phosphatase domain of DUSP5. Methods: pNPP is a mimic of the phosphorylated tyrosine on ...

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Chemistry Central Journal themed issue: Dial-a-Molecule

16-Aug-2015 | Kilpin, Kelly J; Whitby, Richard J, Chemistry Central Journal , 2015

No description available

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Design of symmetric TIM barrel proteins from first principles

12-Aug-2015 | Nagarajan, Deepesh; Deka, Geeta; Rao, Megha, BMC Biochemistry, 2015

Background: Computational protein design is a rapidly maturing field within structural biology, with the goal of designing proteins with custom structures and functions. Such proteins could find widespread medical and industrial applications. Here, we have adapted algorithms from the Rosetta ...

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Promising upshot of silver nanoparticles primed from Gracilaria crassa against bacterial pathogens

07-Aug-2015 | Lavakumar, V; Masilamani, K; Ravichandiran, V; Venkateshan, N; Saigopal, D V R; Ashok Kumar, C K; Sowmya, C, Chemistry Central Journal , 2015

Background: The study on newer antimicrobial agent from metal based nano materials has augmented in recent years for the management of multidrug resistance microorganisms. In our present investigation, we synthesized silver nanoparticles (AgNP’s) from red algae, Gracilaria crassa as beginning ...

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Structural plasticity of green fluorescent protein to amino acid deletions and fluorescence rescue by folding-enhancing mutations

25-Jul-2015 | Liu, Shu-su; Wei, Xuan; Dong, Xue; Xu, Liang; Liu, Jia; Jiang, Biao, BMC Biochemistry, 2015

Background: Green fluorescent protein (GFP) and its derivative fluorescent proteins (FPs) are among the most commonly used reporter systems for studying gene expression and protein interaction in biomedical research. Most commercially available FPs have been optimized for their oligomerization ...

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