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38 Newest Publications about the topic chromatography accessories

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Effects of Bed Compression on Protein Separation on Gel Filtration Chromatography at Bench and Pilot Scale

11-Aug-2017 | Darryl Y. C. Kong, Spyridon Gerontas, Ross A. McCluckie, Martin Mewies, David Gruber, Nigel J. Titchener‐Hooker, Journal of Chemical Technology & Biotechnology, 2017

Abstract BACKGROUND Poorly packed chromatography columns are known to reduce drastically the column efficiency and produce broader peaks. Controlled bed compression has been suggested to be a useful approach for solving this problem. Here the relationship between column efficiency and ...

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Salient Sub-Second Separations

16-Aug-2016 | M. Farooq Wahab; Rasangi M. Wimalasinghe; Yadi Wang; Chandan L. Barhate; Darshan C. Patel; Daniel W. Armstrong, Analytical Chemistry, 2016

Sub-second liquid chromatography in very short packed beds is demonstrated as a broad proof of concept for chiral, achiral, and HILIC separations of biologically important molecules. Superficially porous particles (SPP, 2.7 μm) of different surface chemistries, namely, teicoplanin, cyclofructan, ...

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Isolation and Identification of Potential Allelochemicals from Aerial Parts of Avena fatua L. and Their Allelopathic Effect on Wheat

28-Apr-2016 | Xingang Liu; Fajun Tian; Yingying Tian; Yanbing Wu; Fengshou Dong; Jun Xu; Yongquan Zheng, Journal of Agricultural and Food Chemistry, 2016

Five compounds (syringic acid, tricin, acacetin, syringoside, and diosmetin) were isolated from the aerial parts of wild oats (Avena fatua L.) using chromatography columns of silica gel and Sephadex LH-20. Their chemical structures were identified by means of electrospray ionization and ...

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Achieving 100% Efficient Postcolumn Hydride Generation for As Speciation Analysis by Atomic Fluorescence Spectrometry

14-Mar-2016 | Karel Marschner; Stanislav Musil; Jiří Dědina, Analytical Chemistry, 2016

An experimental setup consisting of a flow injection hydride generator coupled to an atomic fluorescence spectrometer was optimized in order to generate arsanes from tri- and pentavalent inorganic arsenic species (iAsIII, iAsV), monomethylarsonic acid (MAsV), and dimethylarsinic acid (DMAsV) with ...

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High‐resolution magic angle spinning 1H NMR measurement of ligand concentration in solvent‐saturated chromatographic beads

21-Jan-2016 | Fredrik Elwinger, István Furó, Magnetic Resonance in Chemistry, 2016

A method based on 1H high‐resolution magic angle spinning NMR has been developed for measuring concentration accurately in heterogeneous materials like that of ligands in chromatography media. Ligand concentration is obtained by relating the peak integrals for a butyl ligand in the spectrum of a ...

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Aqueous size‐exclusion chromatographic separations of intact proteins under native conditions: Effect of pressure on selectivity and efficiency

09-Nov-2015 | Jelle Vos, Erwin R. Kaal, Remco Swart, Martyna Baca, Yvan Vander Heyden, Sebastiaan Eeltink, Journal of Separation Science, 2015

The selectivity and separation efficiency of aqueous size‐exclusion chromatographic separations of intact proteins were assessed for different flow rates, using columns packed with 3 and 5 μm silica particles containing 150 and 290 Å stagnant pores. A mixture of intact proteins with molecular ...

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13C SPE MAS measurement of ligand concentration in compressible chromatographic beads

06-Jun-2015 | Fredrik Elwinger, Sergey V. Dvinskikh, István Furó, Magnetic Resonance in Chemistry, 2015

A method for measuring the ligand concentration in heterogeneous materials like chromatography media is described. In this method, 13C single pulse excitation magic angle spinning NMR experiment with broadband 1H decoupling is used to determine the peak integrals for a butyl ligand in the ...

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Peptides derivatized with bicyclic quaternary ammonium ionization tags. Sequencing via tandem mass spectrometry

30-Sep-2014 | Bartosz Setner, Magdalena Rudowska, Ewelina Klem, Marek Cebrat, Zbigniew Szewczuk, Journal of Mass Spectrometry, 2014

Improving the sensitivity of detection and fragmentation of peptides to provide reliable sequencing of peptides is an important goal of mass spectrometric analysis. Peptides derivatized by bicyclic quaternary ammonium ionization tags: 1‐azabicyclo[2.2.2]octane (ABCO) or ...

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Highly Stable Surface Functionalization of Microgas Chromatography Columns Using Layer-by-Layer Self-Assembly of Silica Nanoparticles

15-Aug-2013 | Dong Wang; Hamza Shakeel; John Lovette; Gary W. Rice; James R. Heflin; Masoud Agah, Analytical Chemistry, 2013

A controllable and high-yield surface functionalization of silicon microchannels using layer-by-layer (LbL) self-assembly of SiO2 nanoparticles (SNPs) is presented. The application of SNPs (45 nm average diameter) coating as a stationary phase for chromatographic separation is also demonstrated ...

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Optimized Nonlinear Gradients for Reversed-Phase Liquid Chromatography in Shotgun Proteomics

24-Jul-2013 | Luminita Moruz; Peter Pichler; Thomas Stranzl; Karl Mechtler; Lukas Käll, Analytical Chemistry, 2013

Reversed-phase liquid chromatography has become the preferred method for separating peptides in most of the mass spectrometry-based proteomics workflows of today. In the way the technique is typically applied, the peptides are released from the chromatography column by the gradual addition of an ...

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