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13 Newest Publications about the topic electrophoresis gels

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Examination of tryptamine-squaraine complexes as both colorimetric and fluorometric stains in gel electrophoresis

04-Apr-2016 | Author(s): Daniel E. Lynch, Martin J. Cox, Patrick M. King, Graham Smith, Journal of Photochemistry and Photobiology A: Chemistry, 2016

Publication date: 30 June 2016 Source:Journal of Photochemistry and Photobiology A: Chemistry, Volume 324 Author(s): Daniel E. Lynch, Martin J. Cox, Patrick M. King, Graham Smith A water-soluble dual pendent squaraine with tryptaminium cations has been mixed with mono-nitroaromatics ...

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Excited State Dynamics of Brightly Fluorescent Second Generation Epicocconone Analogues

06-May-2015 | Soumit Chatterjee; Peter Karuso; Agathe Boulangé; Xavier Franck; Anindya Datta, Journal of Physical Chemistry B, 2015

The natural product epicocconone, owing to its unique fluorescence properties, has been developed into a range of products used in biotechnology, especially proteomics. However, its weak green fluorescence in its native state, while advantageous for proteomics applications, is a disadvantage in ...

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Properties of nucleic acid staining dyes used in gel electrophoresis

27-Dec-2014 | Alicia M. Haines, Shanan S. Tobe, Hilton J. Kobus, Adrian Linacre, ELECTROPHORESIS, 2014

Nucleic acid staining dyes are used for detecting nucleic acids in electrophoresis gels. Historically the most common dye used for gel staining is ethidium bromide however due to its toxicity and mutagenicity other dyes that are safer to the user and the environment are preferred. This Short ...

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Diced Electrophoresis Gel Assay for Screening Enzymes with Specified Activities

16-Apr-2013 | Toru Komatsu; Kenjiro Hanaoka; Alexander Adibekian; Kentaro Yoshioka; Takuya Terai; Tasuku Ueno; Mitsuyasu Kawaguchi ..., Journal of the American Chemical Society, 2013

Journal of the American Chemical Society DOI: 10.1021/ja401792d

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Characterization of Selenium Incorporation into Wheat Proteins by Two-Dimensional Gel Electrophoresis–Laser Ablation ICP MS followed by capillary HPLC–ICP MS and Electrospray Linear Trap Quadrupole Orbitrap MS

07-Feb-2013 | Juliusz Bianga; Espen Govasmark; Joanna Szpunar, Analytical Chemistry, 2013

A method has been developed for a rapid and precise location of selenium-containing proteins in large two-dimensional (2D) electrophoresis gels. A sample was divided into four aliquots which were analyzed in parallel by 1D isoelectric focusing electrophoresis (IEF)–laser ablation (LA) inductively ...

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Coupling of native IEF and extended x‐ray absorption fine structure to characterize zinc binding sites from pI isoforms of SOD1 and A4V pathogenic mutant

09-Feb-2012 | Chevreux, Sylviane; Llorens, Isabelle; Solari, Pier Lorenzo; Roudeau, Stéphane; Devès, Guillaume; Carm ..., ELECTROPHORESIS, 2012

Extended X‐ray Absorption Fine Structure (EXAFS) has already provided high resolution structures of metal binding sites in a wide variety of metalloproteins. Usually, EXAFS is performed on purified metalloproteins either in solution or crystallized but purification steps are prone to modify the ...

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Coupling of native IEF and extended x‐ray absorption fine structure to characterize zinc binding sites from pI isoforms of SOD1 and A4V pathogenic mutant

09-Feb-2012 | Chevreux, Sylviane; Llorens, Isabelle; Solari, Pier Lorenzo; Roudeau, Stéphane; Devès, Guillaume; Carm ..., ELECTROPHORESIS, 2012

Extended X‐ray Absorption Fine Structure (EXAFS) has already provided high resolution structures of metal binding sites in a wide variety of metalloproteins. Usually, EXAFS is performed on purified metalloproteins either in solution or crystallized but purification steps are prone to modify the ...

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Characterization of proteins transferred from polyacrylamide gels to membrane filters

17-Aug-2011 | Ino, Yoko; Hirano, Hisashi, FEBS Journal, 2011

In the 1990s, a technique was developed to transfer proteins from electrophoresis gels onto PVDF membranes, digest the proteins on the membranes with proteases such as trypsin, and analyze the resulting peptides on the membranes directly by MALDI‐MS to identify the proteins. This technique based ...

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Involvement of ubiquitin‐proteasome system in icariin‐induced cardiomyocyte differentiation of embryonic stem cells using two‐dimensional gel electrophoresis

12-Jul-2011 | Zhu, Dan‐yan; Cui, Rong; Zhang, Ying‐ying; Li, Huan; Zhou, Li‐min; Lou, Yi‐jia, Journal of Cellular Biochemistry, 2011

Abstract Icariin has been shown to significantly facilitate the differentiation of embryonic stem (ES) cells into cardiomyocytes, in vitro. However, the mechanism underlying the icariin‐induced cadiomyocyte differentiation is still not fully understood. In the present study, 52 differentially ...

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Comparative Proteomics Analysis of the Root Apoplasts of Rice Seedlings in Response to Hydrogen Peroxide

10-Feb-2011 | Lu Zhou et al., PLoS ONE, 2011

Background Plant apoplast is the prime site for signal perception and defense response, and of great importance in responding to environmental stresses. Hydrogen peroxide (H2O2) plays a pivotal role in determining the responsiveness of cells to stress. However, how the apoplast ...

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