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107 Newest Publications about the topic fluorescence microscopes

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STED‐TEM Correlative Microscopy Leveraging Nanodiamonds as Intracellular Dual‐Contrast Markers

18-Dec-2017 | Neeraj Prabhakar, Markus Peurla, Sami Koho, Takahiro Deguchi, Tuomas Näreoja, Huan‐Cheng Chang, Jessica M. Rosenholm ..., Small, 2017

Abstract Development of fluorescent and electron dense markers is essential for the implementation of correlative light and electron microscopy, as dual‐contrast landmarks are required to match the details in the multimodal images. Here, a novel method for correlative microscopy that utilizes ...

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Sensors, Vol. 15, Pages 23418-23430: A Simple, Low-Cost Platform for Real-Time Isothermal Nucleic Acid Amplification

16-Sep-2015 | Craw, Pascal ; Mackay, Ruth E.; Naveenathayalan, Angel ; Hudson, Chris ; Branavan, Manoharanehru ; Sadiq, S. Tariq; ..., Sensors, 2015

Advances in microfluidics and the introduction of isothermal nucleic acid amplification assays have resulted in a range of solutions for nucleic acid amplification tests suited for point of care and field use. However, miniaturisation of instrumentation for such assays has not seen such rapid ...

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Complete determination of plant tissues based only on auto‐fluorescence and the advanced image analysis – study of needles and stamens

23-Jul-2015 | Aleksandar G. Savić, Suzana Zivković, Katarina K. Jovanović, Ludovic Duponchel, Ivica Kopriva, Journal of Chemometrics, 2015

Proper determination of tissues is one of the challenging problems in modern medicine and histology. Currently, interpretation of the results mainly depends on the experience of a histologist, leading to high percentage of results misinterpretation. Bearing in mind potential application, we ...

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Monitoring Tectal Neuronal Activities and Motor Behavior in Zebrafish Larvae

01-Sep-2013 | Sumbre, G., Poo, M.-M., CSH Protocols, 2013

To understand how visuomotor behaviors are controlled by the nervous system, it is necessary to monitor the activity of large populations of neurons with single-cell resolution over a large area of the brain in a relatively simple, behaving organism. The zebrafish larva, a small lower vertebrate ...

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Effect of Mechanical Instability of Polymer Scaffolds on Cell Adhesion

20-Aug-2013 | Shinichiro Shimomura; Hisao Matsuno; Keiji Tanaka, Langmuir, 2013

The adhesion of fibroblast on polymer bilayers composed of a glassy polystyrene (PS) prepared on top of a rubbery polyisoprene (PI) was studied. Since the top PS layer is not build on a glassy, or firm, foundation, the system becomes mechanically unstable with decreasing thickness of the PS ...

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Precise Quantification of Transcription Factors in a Surface-based Single-molecule Assay

18-Aug-2013 | Author(s): Kristin S. Grußmayer , Tanja Ehrhard , Konstantinos Lymperopoulos , Dirk-Peter Herten, Biophysical Chemistry, 2013

Publication date: Available online 15 August 2013 Source:Biophysical Chemistry Author(s): Kristin S. Grußmayer , Tanja Ehrhard , Konstantinos Lymperopoulos , Dirk-Peter Herten Biosensors have recognized a rapid development the last years in both industry and science. Recently, a ...

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Hybrid Semiconducting Polymer Nanoparticles as Polarization-Sensitive Fluorescent Probes

29-Jul-2013 | Maxwell B. Zeigler; Wei Sun; Yu Rong; Daniel T. Chiu, Journal of the American Chemical Society, 2013

Much work has been done on collapsed chains of conjugated semiconducting polymers and their applications as fluorescent probes or sensors. On surfaces spin-coated with semiconducting polymers, excitation energy transfer along the polymer backbone can be used to quickly and efficiently funnel ...

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Energy-dependent motion of TonB in the Gram-negative bacterial inner membrane [Microbiology]

09-Jul-2013 | Lorne D. Jordan; Yongyao Zhou; Chuck R. Smallwood; Yoriko Lill; Ken Ritchie; Wai Tak Yip; Salete M. Newton; Phillip ..., Proceedings of the National Academy of Sciences current issue, 2013

Gram-negative bacteria acquire iron with TonB-dependent uptake systems. The TonB–ExbBD inner membrane complex is hypothesized to transfer energy to outer membrane (OM) iron transporters. Fluorescence microscopic characterization of green fluorescent protein (GFP)-TonB hybrid proteins revealed an ...

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Superresolution Multidimensional Imaging with Structured Illumination Microscopy

05-Jul-2013 | Aurélie Jost; Rainer Heintzmann, Annual Review of Materials Research, 2013

The resolution of an optical microscope is fundamentally limited by diffraction. In a conventional wide-field fluorescence microscope, the resolution limit is at best 200 nm. However, modern superresolution methods can bypass this limit. Pointillistic imaging techniques like PALM (photoactivated ...

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The gray institute ‘open’ high‐content, fluorescence lifetime microscopes

12-Jun-2013 | P. R. BARBER, I. D. C. TULLIS, G. P. PIERCE, R. G. NEWMAN, J. PRENTICE, M. I. ROWLEY, D. R. MATTHEWS, S. M. AMEER‐BE ..., Journal of Microscopy, 2013

Summary We describe a microscopy design methodology and details of microscopes built to this ‘open’ design approach. These demonstrate the first implementation of time‐domain fluorescence microscopy in a flexible automated platform with the ability to ease the transition of this and other ...

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