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34 Newest Publications about the topic hydrophobic interaction chromatography


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Mechanistic modeling of hydrophobic interaction chromatography for monoclonal antibody purification: Process optimization in the quality by design paradigm

13-May-2017 | Lalita Kanwar Shekhawat, Monu Chandak, Anurag S. Rathore, Journal of Chemical Technology & Biotechnology, 2017

Abstract BACKGROUND A mechanistic model has been developed for hydrophobic interaction chromatography (HIC) based purification of monoclonal antibodies (mAbs). The model has been used to gain thorough process understanding as desired by the quality by design paradigm. The model is based on ...


On-line determination by small angle X-ray scattering of the shape of hen egg white lysozyme immediately following elution from a hydrophobic interaction chromatography column

20-Aug-2016 | Chadin Kulsing; Andras Z. Komaromy; Reinhard I. Boysen; Milton T. W. Hearn, Analyst, 2016

This study documents the use of an integrated approach, involving on-line hydrophobic interaction chromatography interfaced with Small Angle X-ray Scattering (HIC–SAXS) measurements, to monitor the conformational status of proteins immediately upon elution from a chromatographic column operated ...


Preparation of a silica‐based high‐performance hydrophobic interaction chromatography stationary phase for protein separation and renaturation

09-May-2016 | Yicong Yang, Qian Qu, Weimin Li, Jie Yuan, Yi Ren, Lili Wang, Journal of Separation Science, 2016

Abstract In this work, based on the structural characteristics of bio‐membrane molecules, a novel type of high‐performance hydrophobic interaction chromatography stationary phase was prepared using cholesterol as a ligand. Investigating the separation performance of this stationary phase, the ...


Purification and Characterization of β‐Glucosidase from Brassica oleracea

21-Apr-2016 | Larisa Bešić, Adna Ašić, Imer Muhović, Serkan Dogan, Yusuf Turan, Journal of Food Processing and Preservation, 2016

Abstract β‐Glucosidase was purified from Brassica oleracea by salting out with ammonium sulfate and hydrophobic interaction chromatography. Results demonstrated that the enzyme is a dimer (130 kD) made up of one major (80 kD) and one minor subunit (50 kD). The pH optimum is 6.0, with 50% of ...


Online Hydrophobic Interaction Chromatography–Mass Spectrometry for Top-Down Proteomics

14-Jan-2016 | Bifan Chen; Ying Peng; Santosh G. Valeja; Lichen Xiu; Andrew J. Alpert; Ying Ge, Analytical Chemistry, 2016

Recent progress in top-down proteomics has led to a demand for mass spectrometry (MS)-compatible chromatography techniques to separate intact proteins using volatile mobile phases. Conventional hydrophobic interaction chromatography (HIC) provides high-resolution separation of proteins under ...


Two variables dominating the retention of intact proteins under gradient elution with simultaneous ultrafast high-resolution separation by hydrophobic interaction chromatography

19-Aug-2015 | xindu Geng; Xiaodan JIa; Peng Liu; Fei Wang; Xiaoming Yang, Analyst, 2015

The retention of intact proteins under gradient elution in hydrophobic interaction chromatography (HIC) was found to be governed by two variables of the steady region (SR) and migration region (MR). In the SR, the proteins are immobilized by the strong interactions with the stationary phase such ...


Three Dimensional Liquid Chromatography Coupling Ion Exchange Chromatography/Hydrophobic Interaction Chromatography/Reverse Phase Chromatography for Effective Protein Separation in Top-Down Proteomics

29-Apr-2015 | Santosh G. Valeja; Lichen Xiu; Zachery R. Gregorich; Huseyin Guner; Song Jin; Ying Ge, Analytical Chemistry, 2015

To address the complexity of the proteome in mass spectrometry (MS)-based top-down proteomics, multidimensional liquid chromatography (MDLC) strategies that can effectively separate proteins with high resolution and automation are highly desirable. Although various MDLC methods that can ...


Dysregulation of Interphotoreceptor Retinoid‐Binding Protein (IRBP) after induced Müller Cell disruption

18-Feb-2015 | Ling Zhu, Weiyong Shen, Brian Lyons, Ying Wang, Fanfan Zhou, Mark C. Gillies, Journal of Neurochemistry, 2015

Abstract Reduced expression of a ~150kDa protein was unexpectedly observed while investigating Norrin protein in a transgenic murine model in which Müller cells can be selectively and inducibly disrupted. Isolation of this unknown protein via ion exchange and hydrophobic interaction ...


Preparation of a Weak Anion Exchange/Hydrophobic Interaction Dual‐function Mixed‐Mode Chromatography Stationary Phase for Protein Separation Using Click Chemistry

26-Dec-2014 | Kailou Zhao, Fan Yang, Hongjun Xia, Fei Wang, Qinguo Song, Quan Bai, Journal of Separation Science, 2014

In this study, 3‐diethylamino‐1‐propyne was covalently bonded to the azide‐silica by a click reaction to obtain a novel dual‐function mixed‐mode chromatography stationary phase for protein separation with a ligand containing tertiary amine and two ethyl groups capable of electrostatic and ...


The “Real Environment” Quantification of Surface Hydrophobicity of Differently Stabilized Nanocrystals as Key Parameter for Organ Distribution

16-Dec-2014 | Sven Staufenbiel, Cornelia M. Keck, Rainer H. Müller, Macromolecular Symposia, 2014

Summary The surface hydrophobicity of nanoparticles is one factor determining blood protein adsorption after intravenous administration, thus the organ distribution. Hydrophobic surfaces lead to opsonization and uptake by the liver macrophages, when hydrophilic nanoparticles avoid this and ...


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