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151 Newest Publications about the topic ion-exchange chromatography


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Isolation, purification and characterisation of polygalacturonase from ripened banana (Musa acuminata cv. Kadali)

07-Sep-2013 | Thulasy Gayathri, Ashalatha S. Nair, International Journal of Food Science & Technology, 2013

Summary Bananas are the most important fruit crop in the world. Short shelf life of the fruit is the major limiting factor in international trade, and this is due to the softening of the pulp during ripening. Fruit softening is an important aspect of ripening process in fleshy fruits and is ...


Partial Purification and Characterization of Peroxidase from Pearl Millet (Pennisetum Glaucum [L.] R. Br.) Grains

16-Jul-2013 | Preeti Goyal, Lakshman K. Chugh, Journal of Food Biochemistry, 2013

Abstract Peroxidase from pearl millet hybrid HHB 94 was purified stepwise by (NH4)2SO4 fractionation, gel filtration and ion exchange chromatography using Sephadex G‐100 and DEAE‐cellulose to near homogeneity. The protocol yielded 41% of peroxidase with 33.2‐fold purification. The purified ...


Activities and sequences of the angiotensin I‐converting enzyme (ACE) inhibitory peptides obtained from the digested lentil (Lens culinaris) globulins

12-Jul-2013 | Anna Jakubczyk, Barbara Baraniak, International Journal of Food Science & Technology, 2013

Summary The aim of this study was the identification of potentially bioaccessible ACE‐inhibitory peptides obtained by in vitro gastrointestinal digestion of lentil globulins. ACE‐inhibitory peptides were purified by ion exchange chromatography and gel filtration. After the first step of ...


Mixed-Bed Ion Exchange Chromatography Employing a Salt-Free pH Gradient for Improved Sensitivity and Compatibility in MudPIT

02-Jul-2013 | Geert P. M. Mommen; Hugo D. Meiring; Albert J. R. Heck; Ad P. J. M. de Jong, Analytical Chemistry, 2013

In proteomics, comprehensive analysis of peptides mixtures necessitates multiple dimensions of separation prior to mass spectrometry analysis to reduce sample complexity and increase the dynamic range of analysis. The main goal of this work was to improve the performance of (online) ...


Asparagus Byproducts as a New Source of Peroxidases

18-Jun-2013 | Sara Jaramillo-Carmona; Sergio Lopez; Sara Vazquez-Castilla; Rocio Rodriguez-Arcos; Ana Jimenez-Araujo; Rafael Guill ..., Journal of Agricultural and Food Chemistry, 2013

Soluble peroxidase (POD) from asparagus byproducts was purified by ion exchange chromatographies, and its kinetic and catalytic properties were studied. The isoelectric point of the purified isoperoxidases was 9.1, and the optimum pH and temperature values were 4.0 and 25 °C, respectively. The ...


Ion pairing and ion exchange chromatography coupled to ICP-MS to determine selenium species in human urine

05-Jun-2013 | Thomas Jäger; Hans Drexler; Thomas Göen, Journal of Analytical Atomic Spectrometry, 2013

In this work three analytical methods were developed and applied to determine a broad spectrum of selenium species in human urine. The methods are based on different liquid chromatographic separation techniques (ion pairing, anion exchange and cation exchange chromatography) coupled to an ...


Purification and Characterization of a Temperature- and pH-Stable Laccase from the Spores of Bacillus vallismortis fmb-103 and Its Application in the Degradation of Malachite Green

03-Jun-2013 | Chong Zhang; Shuang Zhang; Hanwen Diao; Haizhen Zhao; Xiaoyu Zhu; Fengxia Lu; Zhaoxin Lu, Journal of Agricultural and Food Chemistry, 2013

Malachite green residue can affect aquaculture food safety. Bioremediation of contaminated water by enzyme treatment is an environmentally friendly and economical way to remove contaminating substances. In the present study, a temperature- and pH-stable laccase was purified from the spores of ...


Trypsin from viscera of vermiculated sailfin catfish, Pterygoplichthys disjunctivus, Weber, 1991: Its purification and characterization

02-Jun-2013 | Author(s): Ana Gloria Villalba-Villalba , Juan Carlos Ramírez-Suárez , Elisa Miriam Valenzuela-Soto , Guillermina ..., Food Chemistry, 2013

Publication date: 15 November 2013 Source:Food Chemistry, Volume 141, Issue 2 Author(s): Ana Gloria Villalba-Villalba , Juan Carlos Ramírez-Suárez , Elisa Miriam Valenzuela-Soto , Guillermina García Sánchez , Gisela Carvallo Ruiz , Ramón Pacheco-Aguilar Pterygoplichthys disjunctivus ...


Conjugation Site Heterogeneity Causes Variable Electrostatic Properties in Fc Conjugates

31-May-2013 | Nicholas J. Boylan; Wen Zhou; Robert J. Proos; Thomas J. Tolbert; Janet L. Wolfe; Jennifer S. Laurence, Bioconjugate Chemistry, 2013

Immunoconjugates, including antibody–drug conjugates and Fc-conjugates, represent a rapidly growing class of therapeutics undergoing clinical development. Despite their growing popularity, the high intrinsic heterogeneity of immunoconjugates often complicates the development process and limits ...


Cell Wall Components of Leptosphaeria maculans Enhance Resistance of Brassica napus

20-May-2013 | Phuong Dinh Kim; Vladimír Šašek; Lenka Burketová; Jana Čopíková; Andriy Synytsya; Barbora Jindřichová; Olga Valentová, Journal of Agricultural and Food Chemistry, 2013

Preparations with elicitation activity were obtained from the mycelium of Leptosphaeria maculans, a fungal pathogen of oilseed rape (Brassica napus). Crude delipidated and deproteinized extract from fungal cell walls induced expression of pathogenesis related gene 1 (PR1), hydrogen peroxide ...


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