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29 Newest Publications about the topic light scattering detectors

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Study of mechanism retention of nonvolatile alkylphosphonic acids on unmodified selected polymer-based ion exchanger columns

10-Jun-2011 | Journal of Analytical Chemistry, 2011

Abstract The retention of alkylphosphonic acids (MPA, EPA, PPA, and BPA) and inorganic anions (Cl−, NO3−, and SO42−) were studied on two anion exchangers, e.g., Dionex IonPac AS4A-SC (250 ± 4 mm I.D.) and Satisfaction P 4000-SAX (50 ± 4.6 mm I.D.) under isocratic elution conditions with ...

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Development of a rapid and convenient method to separate eight ginsenosides from Panax ginseng by high‐speed counter‐current chromatography coupled with evaporative light scattering detection

01-May-2011 | Shehzad, Omer; Jin Ha, In; Park, Youmie; Wan Ha, Young; Shik Kim, Yeong, Journal of Separation Science, 2011

Abstract Ginsenosides exhibit diverse biological activities and are major well‐known components isolated from the radix of Panax ginseng C.A. Meyer. In the present work, a rapid and facile method for the separation and purification of eight ginsenosides from P. ginseng by high‐speed ...

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Enzymatic Modification of a Model Homogalacturonan with the Thermally Tolerant Pectin Methylesterase from Citrus: 1. Nanostructural Characterization, Enzyme Mode of Action, and Effect of pH

02-Mar-2011 | Randall G. Cameron; Gary A. Luzio; Prasanna Vasu; Brett J. Savary; Martin A. K. Williams; Randall G. Cameron; Gary A ..., Journal of Agricultural and Food Chemistry, 2011

Methyl ester distribution in pectin homogalacturonan has a major influence on functionality. Enzymatic engineering of the pectin nanostructure for tailoring functionality can expand the role of pectin as a food-formulating agent and the use of in situ modification in prepared foods. We report on ...

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New Isoflavonoid Glycosides and Related Constituents from Astragali Radix (Astragalus membranaceus) and Their Inhibitory Activity on Nitric Oxide Production

16-Feb-2011 | Li-Jie Zhang; Hui-Kang Liu; Ping-Chun Hsiao; Li-Ming Yang Kuo; I-Jung Lee; Tian-Shung Wu; Wen-Fei Chiou; Yao-Haur Kuo, Journal of Agricultural and Food Chemistry, 2011

Twenty-four secondary metabolites, including 16 isoflavonoids, 7 astragalasides, and 1 benzoquinone, have been isolated from the roots of Astragalus membranaceus (Astragali radix). Among these isolated isoflavonoids, (−)-methylinissolin 3-O-β-d-(6′-acetyl)-glucoside (1), (−)-methylinissolin ...

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Simultaneous Determination of Furostanol, Pennogenyl, and Diosgenyl Glycosides in Taiwanese Rhizoma Paridis (Paris formosana Hayata) by High-Performance Liquid Chromatography with Evaporative Light Scattering Detection

08-Feb-2011 | Jau-Tien Lin; Yan-Zin Chang; Mei-Peng Lu; Deng-Jye Yang, Journal of Agricultural and Food Chemistry, 2011

A high-performance liquid chromatographic method with an evaporative light scattering detector (HPLC−ELSD) was developed to simultaneously determine 10 steroidal saponins, including 3 furostanol glycosides, 3 pennogenyl glycosides, and 4 diosgenyl glycosides in Taiwanese rhizoma paridis (Paris ...

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Validation of a novel LC‐MS/MS method for the quantitation of colistin A and B in human plasma

01-Jan-2011 | Dotsikas, Yannis; Markopoulou, Catherine K.; Koundourellis, John E.; Loukas, Yannis L., Journal of Separation Science, 2011

Abstract A liquid chromatography/tandem mass spectrometry (LC‐MS/MS) method, characterized by complete automation and high‐throughput, was developed for the determination of colistin A and B in human plasma. All sample preparation procedures were performed by using 2.2 mL 96‐deep‐well plates, ...

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Cuticular lipids of insects as potential biofungicides: methods of lipid composition analysis

11-Dec-2010 | Marek Gołębiowski, Mieczysława I. Boguś, Monika Paszkiewicz, Piotr Stepnowski, Analytical and Bioanalytical Chemistry, 2010

The main function of cuticular lipids in insects is the restriction of water transpiration through the surface. Lipids are involved in various types of chemical communication between species and reduce the penetration of insecticides, chemicals, and toxins and they also provide protection from ...

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Hydrodynamic chromatography of latex blends

01-Nov-2010 | Brewer, Amandaa K.; Striegel, André M., Journal of Separation Science, 2010

Abstract The ability to characterize the size and shape distributions of broadly polydisperse analytes is a driving force in particle size analysis. Multi‐detector hydrodynamic chromatography (HDC), which has previously shown promise in its ability to characterize the size and shape of ...

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High-Performance Ion Exchange Chromatography of Intact Bacterial Cells in the Manner of Molecules: 1. Establishment of Methodology

14-Oct-2010 | Shu-tao Liu; Zhao-hua Chen; Jun-bin Xie; Juan Lin; Zhan-jie Chen; Ping-fan Rao, Analytical Chemistry, 2010

The feasibility of separating intact bacterial cells by high-performance ion exchange chromatography typical for macromolecular study was investigated. A common HPLC system coupled with a light scattering detector was employed; TOYOPEARL SuperQ-650C, a beaded anion exchanger, was used as the ...

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An Improved Method for the Separation and Quantification of Major Phospholipid Classes by LC-ELSD

18-Sep-2010 | Kun-Ping Yan, Hong-Li Zhu, Ning Dan, Chao Chen, Chromatographia, 2010

An improved HPLC procedure for the separation of phospholipids is described. The method described utilizes a solvent mixture of acetonitrile-methanol–water-trifluoroacetic acid (100:25:1.7:2.5, v/v) as the mobile phase, which is more compatible with the pump than mobile phases containing ...

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