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33 Newest Publications about the topic multi-angle light scattering

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Characterization of a Protein Conjugate Using an Asymmetrical-Flow Field-Flow Fractionation and a Size-Exclusion Chromatography with Multi-Detection System

17-Aug-2012 | Katja Rebolj; David Pahovnik; Ema Žagar, Analytical Chemistry, 2012

In this study we present detailed characterization of a protein-PEG conjugate using two separation techniques, that is, asymmetrical-flow field-flow fractionation (AF4) and size-exclusion chromatography (SEC), which were online coupled to a series of successively connected detectors: an ...

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Anomalous Small-Angle X-ray Scattering Study of Structure of Polymer Micelles Having Bromines in Hydrophobic Core

23-Jul-2012 | Isamu Akiba; Atsuro Takechi; Megumi Sakou; Masashi Handa; Yuya Shinohara; Yoshiyuki Amemiya; Naoto Yagi; Kazuo Sakurai, Macromolecules, 2012

Anomalous small-angle X-ray scattering (ASAXS) near the absorption edge of bromine has been used to investigate the internal structure of polymer micelles comprising poly(4-bromostyrene)-block-poly(ethylene glycol)-block-poly(4-bromostyrene) (PBrS-b-PEG-b-PBrS) in aqueous solution. The ASAXS ...

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Conformational Characterization of Synapse-Associated Protein 97 by Nuclear Magnetic Resonance and Small-Angle X-ray Scattering Shows Compact and Elongated Forms

19-Jan-2012 | Mark D. Tully; J. Günter Grossmann; Marie Phelan; Sravan Pandelaneni; Mark Leyland; Lu-Yun Lian, Biochemistry, 2012

Synapse-associated protein 97 (SAP97) is a membrane-associated guanylate kinase protein that interacts with other proteins such as ion channels, subunits of glutamate receptors, and other cytoskeletal proteins and molecular scaffolds. The molecular diversity of SAP97 results from alternative ...

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Size‐exclusion chromatography coupled to multiangle light scattering detection of long‐chain branching in polyethylene made with phillips catalyst

21-Dec-2011 | Yu, Youlu; Schwerdtfeger, Eric; McDaniel, Max, Journal of Polymer Science Part A: Polymer Chemistry, 2011

Abstract Size‐exclusion chromatography coupled to multiangle light scattering (SEC‐MALS) has been used to detect long‐chain branching (LCB) in polyethylene (PE) from Cr/silica catalysts for the first time. The observed LCB response to several catalyst and reactor variables mostly confirms ...

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Characterization of sodium hyaluronate blends using frit inlet asymmetrical flow field-flow fractionation and multiangle light scattering

20-Nov-2011 | Muhammad Ali, Euijin Hwang, Il-Hwan Cho, Myeong Hee Moon, Analytical and Bioanalytical Chemistry, 2011

We characterized ultrahigh molecular weight sodium hyaluronate (NaHA) and blended pharmaceutical products containing NaHA using flow field-flow fractionation and multiangle light scattering–differential refractive index (FlFFF-MALS-DRI). NaHA is a water-soluble polysaccharide with a range of ...

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Tunable Encapsulation of Proteins within Charged Microgels

18-Oct-2011 | Michael H. Smith; L. Andrew Lyon, Macromolecules, 2011

The binding of cytochrome c to pH and thermoresponsive colloidal hydrogels was investigated using multiangle light scattering, measuring loading through changes in particle molar mass and root-mean-square radius. Loosely cross-linked microgels [composed of a random copolymer of ...

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Mechanistic study of Atom Transfer Radical Polymerization in the Presence of an Inimer: Toward Highly Branched Controlled Macromolecular Architectures through One-Pot Reaction

24-Aug-2011 | Florence Bally; Esma Ismailova; Cyril Brochon; Christophe A. Serra; Georges Hadziioannou, Macromolecules, 2011

Highly branched polymethacrylates have recently offered new perspectives in lithographic performance and drug delivery. The control of branching remains yet challenging and requires fundamental investigation to consider new applications. Therefore, an advanced study of the formation mechanism of ...

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Network Deconstruction Reveals Network Structure in Responsive Microgels

22-Mar-2011 | Michael H. Smith; Emily S. Herman; L. Andrew Lyon, Journal of Physical Chemistry B, 2011

Detailed characterization of hydrogel particle erosion revealed critical physicochemical differences between spheres, where network decomposition was informative of network structure. Real-time, in situ monitoring of the triggered erosion of colloidal hydrogels (microgels) was performed via ...

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Amyloid Fibrils Formation of Concanavalin A at Basic pH

10-Mar-2011 | R. Carrotta; V. Vetri; F. Librizzi; V. Martorana; V. Militello; M. Leone; R. Carrotta; V. Vetri; F. Librizzi; V. Mar ..., Journal of Physical Chemistry B, 2011

Mechanisms of partial unfolding and aggregation of proteins are of extreme interest in view of the fact that several human pathologies are characterized by the formation and deposition of protein-insoluble material, mainly composed of amyloid fibrils. Here we report on an experimental study on ...

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Quantitative Characterization of Gold Nanoparticles by Field-Flow Fractionation Coupled Online with Light Scattering Detection and Inductively Coupled Plasma Mass Spectrometry

28-Feb-2011 | Bjørn Schmidt; Katrin Loeschner; Niels Hadrup; Alicja Mortensen; Jens J. Sloth; Christian Bender Koch; Erik H. ..., Analytical Chemistry, 2011

An analytical platform coupling asymmetric flow field-flow fractionation (AF4) with multiangle light scattering (MALS), dynamic light scattering (DLS), and inductively coupled plasma mass spectrometry (ICPMS) was established and used for separation and quantitative determination of size and mass ...

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