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109 Newest Publications about the topic sample handling

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Rapid Sample Preparation Methodology for Plant N-Glycan Analysis Using Acid-Stable PNGase H+

23-Nov-2015 | Ya M. Du; Tian Xia; Xiao Q. Gu; Ting Wang; Hong Y. Ma; Josef Voglmeir; Li Liu, Journal of Agricultural and Food Chemistry, 2015

The quantification of potentially allergenic carbohydrate motifs of plant and insect glycoproteins is increasingly important in biotechnological and agricultural applications as a result of the use of insect cell-based expression systems and transgenic plants. The need to analyze N-glycan ...

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Gas phase silanization for silicon nanowire sensors and other lab‐on‐a‐chip systems

19-Nov-2015 | Annina M. Steinbach, Tanja Sandner, Boris Mizaikoff, Steffen Strehle, physica status solidi (c), 2015

Abstract Designing a microfabrication process for lab‐on‐a‐chip systems can at times be challenging, and the need to integrate a chemical surface modification reaction into this process can limit the options. Therefore, a robust set‐up and protocol for the gas phase modification has been ...

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Disposable bacterial lysis cartridge (BLC) suitable for in-situ water-borne pathogen detection system

07-Oct-2015 | Eun-Hee Lee; Hyun Jeong Lim; Ahjeong Son; Beelee Chua, Analyst, 2015

We constructed a disposable bacterial lysis cartridge (BLC) suitable for in-situ pathogen detection system. It had an in-built micro corona discharge based ozone generator that provided the ozone for cell lysis. Using custom sample handling platform, its performance was evaluated with a ...

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Identification of bacteria by fatty acid profiling with direct analysis in real time mass spectrometry

28-Sep-2015 | Robert B. Cody, Casey R. McAlpin, Christopher R. Cox, Kirk R. Jensen, Kent J. Voorhees, Rapid Communications in Mass Spectrometry, 2015

Rationale Bacterial fatty acid profiling is a well‐established technique for bacterial identification. Current methods involving esterification and gas chromatography/mass spectrometry (GC/MS) or matrix‐assisted laser desorption/ionization (MALDI) analysis are effective, but there are ...

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A robust capillary liquid chromatography/tandem mass spectrometry method for quantitation of neuromodulatory endocannabinoids

04-Sep-2015 | Ming Qi, Maria Morena, Haley A. Vecchiarelli, Matthew N. Hill, David C. Schriemer, Rapid Communications in Mass Spectrometry, 2015

Rationale Methods for quantifying anandamide (AEA) and 2‐arachidonoyl glycerol (2‐AG) are needed to support programs investigating molecular mechanisms of the central nervous system. Existing methods, while useful, are not well adapted to efficiently process large numbers of very small tissue ...

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Precise small volume sample handling for capillary electrophoresis

03-Sep-2015 | Mona Mozafari, Markus Nachbar, Sami El Deeb, ELECTROPHORESIS, 2015

Capillary electrophoresis is one of the most important analytical techniques. Although the injected sample volume in capillary electrophoresis is only in the nanoliter range, most commercial CE‐instruments need approximately 50 μL of the sample in the injection vial to perform the analysis. ...

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Flow‐Synthesis of Nucleosides Catalyzed by an Immobilized Purine Nucleoside Phosphorylase from Aeromonas hydrophila: Integrated Systems of Reaction Control and Product Purification

03-Aug-2015 | Enrica Calleri, Giulia Cattaneo, Marco Rabuffetti, Immacolata Serra, Teodora Bavaro, Gabriella Massolini, Giovanna S ..., Advanced Synthesis & Catalysis, 2015

Abstract A purine nucleoside phosphorylase from Aeromonas hydrophyla (AhPNP) was covalently immobilized in a pre‐packed stainless steel column containing aminopropylsilica particles via Schiff base chemistry upon glutaraldehyde activation. The resulting AhPNP‐IMER (Immobilized Enzyme Reactor, ...

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In situ X‐ray diffraction environments for high‐pressure reactions

30-Jul-2015 | Bjarne R. S. Hansen, Kasper T. Møller, Mark Paskevicius, Ann‐Christin Dippel, Peter Walter, Colin James Webb, Claudi ..., Journal of Applied Crystallography, 2015

New sample environments and techniques specifically designed for in situ powder X‐ray diffraction studies up to 1000 bar (1 bar = 105 Pa) gas pressure are reported and discussed. The cells can be utilized for multiple purposes in a range of research fields. Specifically, investigations of ...

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Computer simulations of sample preconcentration in carrier‐free systems and isoelectric focusing in microchannels using simple ampholytes

02-Jun-2015 | Saara Mikkonen, Wolfgang Thormann, Åsa Emmer, ELECTROPHORESIS, 2015

In this work, electrophoretic preconcentration of protein and peptide samples in microchannels was studied theoretically using the 1D dynamic simulator GENTRANS, and experimentally combined with MS. In all configurations studied, the sample was uniformly distributed throughout the channel before ...

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Crystal structures of increasingly large molecules: meeting the challenges with CRYSTALS software

24-May-2015 | Parois, Pascal; Cooper, Richard I; Thompson, Amber L, Chemistry Central Journal , 2015

Background: The size and complexity of molecules being studied by single crystal diffraction is growing year by year, resulting in an increase in the difficulties encountered during structure determination. From the crystallisation itself and sample handling, to structure solution and refinement, ...

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