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3 Newest Publications about the topic uhplc systems

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Sugar nucleotide quantification using multiple reaction monitoring liquid chromatography/tandem mass spectrometry

26-Jun-2013 | Aldo D. Garcia, Jorge L. Chavez, Yehia Mechref, Rapid Communications in Mass Spectrometry, 2013

RATIONALE Glycosylation of proteins and lipids is reliant on the availability of monosaccharide‐activated donors known as sugar nucleotides. They are responsible for glycosylation in cells. Reliable quantification of these sugar nucleotides might provide an insight into their biological roles ...

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Separation and Identification of Individual Alginate Oligosaccharides in the Feces of Alginate-Fed Pigs

10-Jan-2013 | Melliana C. Jonathan; Guido Bosch; Henk A. Schols; Harry Gruppen, Journal of Agricultural and Food Chemistry, 2013

This research aimed to develop a method for analyzing specific alginate oligosaccharides (AOS) in a complex matrix such as pig feces. The data obtained were used to study alginate degradation by the microbiota in the large intestine during adaptation, including the individual variation between ...

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In-house construction of a UHPLC system enabling the identification of over 4000 protein groups in a single analysis

24-May-2012 | Alba Cristobal; Marco L Hennrich; Piero Giansanti; Soenita S G; A J R Heck; Shabaz Mohammed, Analyst, 2012

Here, we describe an in-house built ultra-high pressure liquid chromatography (UHPLC) system, with little complexity in design and high separation power combined with convenience in operation. This system enables the use of long columns of 40 cm packed with 1.8 µm particles generating pressures ...

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