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The initial stage of the photosynthetic system is the light-dependent reaction, which converts solar energy into chemical energy.
The light dependent reaction produces oxygen gas and converts ADP and NADP+ into the energy carriers ATP and NADPH.
The first ideas about light being used in photosynthesis were proposed by Jan Ingenhousz in 1779who recognised it was sunlight falling on plants that was required, although Joseph Priestly had noted the production of oxygen without the association with light in 1772. Cornelius Van Niel proposed in 1931 that photosynthesis is a case of general mechanism where a photon of light is used to photodecompose a hydrogen donor and the hydrogen being used to reduce CO2. Then in 1939 Robin Hill showed that isolated chloroplasts would make oxygen, but not fix CO2 showing the light and dark reactions occurred in different places. This lead later to the discovery of photosystem 1 and 2.
The chlorophyll's electron can follow either of two different pathways, cyclic or non-cyclic.
In cyclic electron flow, the electron begins in a pigment complex called photosystem I, passes from the primary acceptor to ferredoxin, then to a complex of two cytochromes (similar to those found in mitochondria), and then to plastocyanin before returning to chlorophyll. This transport chain produces a proton-motive force, pumping H+ ions across the membrane; this produces a concentration gradient which can be used to power ATP synthase during chemiosmosis. This pathway is known as cyclic photophosphorylation, and it produces neither O2 nor NADPH. In bacterial photosynthesis, a single photosystem is used, and therefore is involved in cyclic photophosphorylation.
The other pathway, noncyclic photophosphorylation, is a two-stage process involving two different chlorophyll photosystems. First, a water molecule is broken down into 2H+ + 1/2O2 + 2e- by a process called photolysis (or light-splitting). The two electrons from the water molecule are kept in photosystem II, while the 2H+ and 1/2O2 are left out for further use. Then a photon is absorbed by the chlorophyll core of photosystem II, exciting the two electrons which are transferred to the acceptor molecule. The deficit of electrons is replenished by taking electrons from another molecule of water. The electrons transfer from the primary acceptor to plastoquinone, then to plastocyanin, producing proton-motive force as with cyclic electron flow and driving ATP synthesis.
The photosystem II complex replaced its lost electrons from an external source, however, the two other electrons are not returned to photosystem II as they would in the analogous cyclic pathway. Instead, the still-excited electrons are transferred to a photosystem I complex, which boosts their energy level to a higher level using a second solar photon. The highly excited electrons are transferred to the acceptor molecule, but this time are passed on to an enzyme called Ferredoxin- NADP reductase|NADP+ reductase, for short FNR, which uses them to catalyst the reaction (as shown):
This consumes the H+ ions produced by the splitting of water, leading to a net production of 1/2O2, ATP, and NADPH+H+ with the consumption of solar photons and water.
The concentration of NADPH in the chloroplast may help regulate which pathway electrons take through the light reactions. When the chloroplast runs low on ATP for the Calvin cycle, NADPH will accumulate and the plant may shift from noncyclic to cyclic electron flow.
It is important to note that both photosystems are almost simultaneously excited; thus, both photosystems begin functioning at almost the same time.
|This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Light-dependent_reaction". A list of authors is available in Wikipedia.|