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Centrifugation is a process that involves the use of the centripetal force for the separation of mixtures, used in industry and in laboratory settings. In chemistry and biology, increasing the effective gravitational force on a test tube so as to more rapidly and completely cause the precipitate ("pellet") to gather on the bottom of the tube. The remaining solution is properly called the "supernate" or "supernatant liquid". Since "supernatant" is an adjective, its usage alone is technically incorrect, although many examples can be found in scientific literature. The supernatant liquid is then either quickly decanted from the tube without disturbing the precipitate, or withdrawn with a Pasteur pipette. The rate of centrifugation is specified by the acceleration applied to the sample, typically measured in revolutions per minute (RPM) or g. The particles' settling velocity in centrifugation is a function of their size and shape, centrifugal acceleration, the volume fraction of solids present, the density difference between the particle and the liquid, and the viscosity.
Additional recommended knowledge
In the chemical and food industries, special centrifuges and can process a continous stream of particle-laden liquid.
There are various types of centrifugation:
Categories: Laboratory techniques | Unit operations
|This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Centrifugation". A list of authors is available in Wikipedia.|